A kind of extraction and separation and purification method of diosgenin from chrysanthemum leaf

A technology of Dioscorea officinalis and Dioscorea saponin is applied in the field of extraction, separation and purification of Dioscorea officinalis saponins, and can solve the problem of no record, etc., and achieve the effects of efficient extraction, high yield and high purity.

Active Publication Date: 2021-08-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no record on how to extract and prepare high-yield total saponins and dioscin from Dioscorea chrysanthemum at present, and detailed research is still needed

Method used

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  • A kind of extraction and separation and purification method of diosgenin from chrysanthemum leaf
  • A kind of extraction and separation and purification method of diosgenin from chrysanthemum leaf
  • A kind of extraction and separation and purification method of diosgenin from chrysanthemum leaf

Examples

Experimental program
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Effect test

Embodiment 1

[0049] The separation of total saponins in Dioscorea chrysanthemum leaf in embodiment 1

[0050] The main saponins in Dioscorea chrysanthemum are 4 kinds of saponins, i.e. diosgenin, methyl protodioscin, saponin Pb and prosaponin Pb, so the total saponins in Dioscorea chrysanthemum are limited to these 4 kinds of saponins, and the following examples are for optimizing separation Process, obtain the high yield method of these 4 kinds of saponins.

[0051] 1. Alcoholic extract from Dioscorea chrysanthemum leaf

[0052] Weigh 960.0 g of diosminum chrysanthemum freeze-dried powder, put it in a 5.0 L Erlenmeyer flask, add 3.0 L of 90% ethanol, soak at room temperature for 24 hours, repeat the extraction three times with the same volume of 90% ethanol, and filter to obtain the extract.

[0053] 2. Determination of saponin content:

[0054] (1) Use saponin perchloric acid color method for determination, the specific process is as follows: use a 200 μL pipette gun to take 200 μL of th...

Embodiment 2

[0084] The main steroidal saponins in Dioscorea chrysanthemum are: diosgenin, methylprotodioscin, saponin Pb and prosenoside Pb. In the following examples, these four steroidal saponins are mainly used as research objects.

[0085] 1. Extraction and separation of diosgenoid saponin from Chrysanthemum leaf

[0086] Weigh 960.0 g of diosminum chrysanthemum freeze-dried powder, put it in a 5.0 L Erlenmeyer flask, add 3.0 L of 90% ethanol, soak at room temperature for 24 hours, repeat the extraction three times with the same volume of 90% ethanol, and filter to obtain the extract. Concentrate the extract with a rotary evaporator at 40°C until there is no alcohol smell, and follow the Figure 7 process is separated.

[0087] Figure 7 Among the final separated substances, 3 represents diosgenin; 5 represents methyl protodioscin; 4 represents saponin Pb; 7 represents protodioscin Pb.

[0088] The concentrated substance of the ethanol extract obtained by the above extraction is di...

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Abstract

The invention discloses a method for extracting, separating and purifying diosgenin from chrysanthemum leaves. The total saponins include diosgenin, protodioscin methyl, saponin Pb and protodioscin Pb, and the specific extraction method is: the tuber of Dioscorea chrysanthemum leaf is washed, dried naturally, sliced, then freeze-dried, crushed into powder, and passed Sieve, then soak the diosminum chrysanthemum freeze-dried powder in ethanol for extraction, and carry out microwave treatment to obtain the extract of total saponins. The present invention extracts the total saponins in Dioscorea chrysanthemum with microwave-assisted extraction technology, and realizes the efficient extraction of total saponins in Dioscorea chrysanthemum by optimizing the microwave extraction conditions, and the yield of total saponins is 8.93 mg / g; at the same time, by adopting a specific stationary phase Column chromatography and gradient elution of the mobile phase separated and obtained 4 steroidal saponins, not only with high yield, but also with contents of 61.1mg / g, 4.5mg / g, 39.9mg / g and 0.84mg / g respectively , and the separation process is simple and fast, the separation effect is good, and the separated saponin has high purity.

Description

technical field [0001] The invention relates to the technical field of separation and extraction of saponins from Dioscorea chrysanthemum leaves, and more specifically, to a method for extracting, separating and purifying dioscin from chrysanthemum leaves. Background technique [0002] Dioscorea composita Hemsl, native to Mexico, has high dioscin and starch content, and is an important plant resource for producing dioscin in America. The steroidal saponins in Dioscorea chrysanthemi have stable components, high yield, obvious planting advantages, and high comprehensive utilization value. Study the utilization technology of Dioscorea chrysanthemum at different levels, improve the processing system of Dioscorea chrysanthemum, increase the utilization channels of Dioscorea chrysanthemum plant resources, improve the comprehensive utilization efficiency of resources, and provide technologies for promoting the comprehensive utilization and industrialization of Dioscorea chrysanthem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00
CPCC07J71/00
Inventor 谢君叶广英毕桂灿
Owner SOUTH CHINA AGRI UNIV
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