MiRNA (micro ribonucleic acid) probe primary screening method and system based on chain replacement reactions

Abstract

The invention belongs to the field of non-encoded RNA (ribonucleic acid) detection, in particular relates to an miRNA (micro ribonucleic acid) probe primary screening method and system based on chainreplacement reactions, and aims to solve the problems that a probe based on artificial experiments is low in designing and screening efficiency and low in accuracy rate. The method comprises the following steps: acquiring a nucleotide sequence to be detected, wherein an input sequence consists of toehold at a 5'-end of a nucleotide sequence and a protection sequence; forming toehold of a 3'-end randomly on the basis of four nucleotides A, T, G and C, connecting the toehold with the protection sequence so as to generate a probe sequence, performing chain replacement reactions through a signal amplification model, and acquiring a reactant sequence and a product sequence of each step of the chain replacement reactions; sequentially calculating molar Gibbs free energy of each step of the chainreplacement reactions; and outputting probe sequences of which the molar Gibbs free energy of each step of the chain replacement reactions is smaller than 0 as a screening result. By adopting the signal amplification model of the chain replacement reactions, the probe screening efficiency and accuracy are greatly improved.

Description

technical field [0001] The invention belongs to the field of non-coding RNA detection, and in particular relates to a method and system for preliminary screening of miRNA probes based on strand displacement reaction. Background technique [0002] At present, non-coding RNAs have been identified as carcinogenic drivers or tumor suppressors of various major cancer types, and the detection of certain specific non-coding RNAs in the body or the inhibition of their expression is of great significance for the diagnosis and treatment of certain diseases, so the design and screening of Efficient and specific probes are the key issues to be solved urgently. [0003] At this stage, the design and screening of probes mainly rely on experiments. At the same time, experiments require multiple sets of repeated experiments and comparative experiments to obtain credible results. However, because the probes were not initially screened before the experiment, the experimental results Often un...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above-mentioned problems in the prior art, that is, in order to solve the problems of low efficiency and accuracy of the existing probe design and screening based on manual experiments, the first aspect of the present invention proposes a miRNA probe based on strand displacement reaction The primary screening method, which includes:

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