Fluorescence immunoassay method for simultaneously detecting tyramine and histamine

A fluorescence immunity, tyramide technology, applied in fluorescence/phosphorescence, measurement device, material analysis by optical means, etc., can solve the problems of poor stability of derivatized substances, high detection cost of large-scale equipment, complicated processing, etc., to achieve detection efficiency Improved, practical, high-sensitivity and rapid detection effects

Active Publication Date: 2020-06-05
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the purpose of the present invention is to provide a fluorescent immunoassay for simultaneous detection of tyramine and histamine, which solves the problem that traditional rapid enzyme immunoassays can only detect a single...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescence immunoassay method for simultaneously detecting tyramine and histamine
  • Fluorescence immunoassay method for simultaneously detecting tyramine and histamine
  • Fluorescence immunoassay method for simultaneously detecting tyramine and histamine

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] The invention utilizes tyramide antibody to modify activated carboxyl functionalized blue-light-emitting water-soluble upconverting nanoparticles to obtain tyramide antibody signal probes. The present invention has no special limitation on the source and preparation method of the tyramide antibody. Preferably, the tyramide-coupled bovine serum albumin is used as the immunogen, and the serum containing the tyramide polyclonal antibody is obtained by immunizing New Zealand white rabbits, and separated and purified. income. The preparation method of the activated carboxyl-functionalized blue-light emitting water-soluble upconversion nanoparticles according to the present invention preferably comprises: mixing yttrium acetate tetrahydrate, ytterbium acetate tetrahydrate and thulium acetate tetrahydrate in a molar ratio of 78-90:9-12 : After 1 mixing, prepare the oil-soluble up-conversion nanomaterial (its transmission electron microscope characterization as figure 2 As sh...

Embodiment 1

[0056] (1) 12 μg of tyramine antibody (tyramine antibody uses tyramine-coupled bovine serum albumin as the immunogen, and the serum containing tyramine polyclonal antibody is obtained by immunizing New Zealand white rabbits, obtained by separation and purification) to modify 250 μg of the activated carboxyl group Functionalized blue light-emitting water-soluble up-conversion nanoparticles (from yttrium acetate tetrahydrate: ytterbium acetate tetrahydrate: thulium acetate tetrahydrate molar ratio is 90:9:1, oil-soluble up-conversion nanomaterials were prepared first, and then the Surface modification of carboxyl groups to prepare water-soluble up-conversion nanomaterials), 50 μg of histamine antibody (a monoclonal antibody obtained by hybridoma technology for histamine antibodies) modified 250 μg of activated carboxyl functionalized green light-emitting water-soluble up-conversion nanoparticles ( The molar ratio of yttrium acetate tetrahydrate: ytterbium acetate tetrahydrate: er...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Volumeaaaaaaaaaa
Login to view more

Abstract

The invention provides a fluorescence immunoassay method for simultaneously detecting tyramine and histamine, and relates to the technical field of fluorescence immunoassay. The method comprises stepsof marking tyramine and histamine specific antibodies by using the up-conversion nanoparticles as fluorescent markers respectively to prepare signal probes; utilizing the specific recognition effectof the antibody-antigen and coating to-be-detected substances tyramine and histamine in the sample and the magnetic polystyrene microspheres with antigens; competitively combining corresponding tyramine and histamine specific antibodies on up-conversion nanoparticles in the signal probe so as to prepare an immune complex; and obtaining an immune complex mixture by virtue of a magnetic separation effect. Signal peaks of tyramine and histamine are respectively represented by characteristic emission peaks at 483nm and 550nm under the excitation of 980nm laser, so as to realize high-specificity and high-sensitivity simultaneous detection of various target objects in a mixed system.

Description

technical field [0001] The invention belongs to the technical field of fluorescent immunoassay, and in particular relates to a fluorescent immunological method for simultaneously detecting tyramine and histamine. Background technique [0002] Biogenic amines are widely found in meat and its products, aquatic products, and fermented foods. Adequate intake of biogenic amines can promote growth, enhance metabolic activity, and enhance immunity, but excessive intake of biogenic amines can cause headaches, abdominal cramps, and vomiting. Adverse physiological reactions, severe and even lead to poisoning death. The United States stipulates that the histamine content in aquatic products shall not exceed 50mg / kg; Australia and Switzerland stipulate that the histamine content in wine shall not exceed 10mg / L, France shall not exceed 8mg / L, and the Netherlands shall not exceed 3.5mg / L. However, Germany is more stringent, and the regulation should not be higher than 2mg / L; the European...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/546G01N33/543G01N21/64
CPCG01N33/54326G01N33/54313G01N21/6428G01N2021/6439G01N2021/6417
Inventor 生威张彪王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap