Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method and application of a hemostatic material based on Antarctic krill shell

An Antarctic krill and hemostatic material technology, applied in the field of medical materials, can solve the problems of wound surface adhesion, poor antibacterial effect, etc., and achieve good antibacterial effect.

Active Publication Date: 2021-10-22
沈阳米尔生物科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention overcomes the defect that the hemostatic material in the prior art is easy to bond with the wound surface and has poor antibacterial effect, and provides a kind of antibacterial material based on Antarctic krill shell that can prevent adhesion with the wound surface and has good antibacterial effect at the same time. Preparation method and application of hemostatic material

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of a hemostatic material based on Antarctic krill shell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of a hemostatic material based on Antarctic krill shells, said method comprising the following steps:

[0033] (1) Take the Antarctic krill shell, firstly immerse it in 4% HCl solution to decalcify it, wash it with water, deproteinize it with 5% dilute NaOH solution, filter it with water and pass it through KMnO with a mass fraction of 0.5% at 40°C 4 The solution was soaked for 30 minutes to decolorize, and finally the concentrated NaOH solution with a mass fraction of 30% was stirred and reacted at 30°C for 2 hours to deacetylate to obtain Antarctic krill chitosan.

[0034] (2) Add Antarctic krill chitosan into 5% hydrogen peroxide solution according to the molar ratio of 1:1, adjust the pH to 5, stir for 1 hour, and freeze-dry to obtain Antarctic krill chitosan.

[0035] (3) Antarctic krill chitooligosaccharide and sodium alginate were dissolved in deionized water respectively to obtain a mass fraction of 3% Antarctic krill chitooligosaccharide so...

Embodiment 2

[0037] A preparation method of a hemostatic material based on Antarctic krill shells, said method comprising the following steps:

[0038] (1) Take the Antarctic krill shell, firstly immerse it in 6% HCl solution to decalcify it, wash it with water, deproteinize it with 10% dilute NaOH solution, filter it with water and pass it through KMnO with a mass fraction of 3% at 75°C 4 The solution was soaked for 60 minutes to decolorize, and finally the concentrated NaOH solution with a mass fraction of 45% was stirred and reacted at 50°C for 5 hours to deacetylate to obtain Antarctic krill chitosan.

[0039] (2) Add Antarctic krill chitosan into 10% hydrogen peroxide solution according to the molar ratio of 1:1.5, adjust the pH to 6, stir for 3 hours, freeze-dry to obtain Antarctic krill chitosan.

[0040] (3) Antarctic krill chitooligosaccharide and sodium alginate were dissolved in deionized water respectively to obtain an Antarctic krill chitooligosaccharide solution with a mass f...

Embodiment 3

[0042] A preparation method of a hemostatic material based on Antarctic krill shells, said method comprising the following steps:

[0043](1) Take the Antarctic krill shell, firstly immerse it in 5% HCl solution to decalcify it, wash it with water, deproteinize it with 6% dilute NaOH solution, filter it with water and pass it through KMnO with a mass fraction of 1.5% at 55°C 4 The solution was soaked for 40 minutes to decolorize, and finally the concentrated NaOH solution with a mass fraction of 35% was stirred and reacted at 40°C for 3 hours to deacetylate to obtain Antarctic krill chitosan.

[0044] (2) Antarctic krill chitosan was added to 8% hydrogen peroxide solution according to the molar ratio of 1:1.2, the pH was adjusted to 5.5, stirred for 2 hours, and freeze-dried to obtain Antarctic krill chitosan.

[0045] After the Antarctic krill chitosan oligosaccharide is prepared, surface treatment is also carried out, and the treatment method is as follows:

[0046] (a) Acc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of medical materials, in particular to a preparation method and application of an Antarctic krill shell-based hemostatic material. The method comprises the following steps: (1) taking Antarctic krill shells, and sequentially decalcifying, deproteinizing, decolorizing and deacetylating to obtain Antarctic krill chitosan; (2) degrading Antarctic krill chitosan Antarctic krill chitosan oligosaccharides are obtained; (3) Antarctic krill chitosan oligosaccharides are cross-linked with sodium alginate solution to obtain a composite gel. The invention overcomes the defect that the hemostatic material in the prior art is easy to bond to the wound surface and has poor antibacterial effect, and can effectively stop bleeding from the wound surface in a short time without bonding to the wound surface. It also has good antibacterial effect.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a preparation method and application of an Antarctic krill shell-based hemostatic material. Background technique [0002] The normal hemostasis of the body mainly depends on the complete structure and function of the blood vessel wall, the quality and quantity of effective platelets, and the normal activity of coagulation factors, among which the role of negatively charged platelets and coagulation factors is the main one. [0003] Generally speaking, when the human body is subjected to a small trauma, the platelets and coagulation factors in the blood can take effect immediately, thereby playing the role of hemostasis in a short time. However, when the wound surface is large, hemostasis cannot be achieved in a short period of time through the body's own blood coagulation function. Therefore, when the wound surface is large, it is necessary to use medical hemostatic materials to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/00A61L26/00
CPCA61L24/0015A61L24/0031A61L24/0042A61L24/0094A61L26/0066A61L26/008A61L26/009A61L26/0095A61L2300/104A61L2300/236A61L2300/404A61L2300/604A61L2400/04C08L5/08C08L5/04
Inventor 欧阳小琨卢雨清杨立业范丽红
Owner 沈阳米尔生物科技有限公司