Honeycomb carrier for culturing hepatocytes in vitro and bioreactor for artificial liver

Abstract

Embodiments of the present application disclose a honeycomb carrier plate for in-vitro culture of hepatocytes and a bioreactor for an artificial liver, and aim to solve the problems of insufficient space for agglomeration growth of extracorporeal hepatocytes and low substance exchange efficiency in a bioartificial liver. According to the honeycomb carrier plate for the in-vitro culture of the hepatocytes provided by the embodiments of the present application, honeycomb holes in the honeycomb carrier plate are divided into first honeycomb holes and second honeycomb holes, the first honeycomb holes are opened at the top ends and closed at the bottom ends, and are used for accommodating the hepatocytes, and the second honeycomb holes are closed or opened at the top ends and opened at the bottom ends; and the honeycomb wall of each honeycomb hole is provided with a first through hole with a pore diameter smaller than the diameter of each hepatocyte, the closed ends of the first honeycomb holes and the second honeycomb holes are provided with second through holes with a pore diameter smaller than the diameter of each hepatocyte, each second honeycomb hole is arranged in same edges withsix first honeycomb holes adjacent to the second honeycomb hole, and each first honeycomb hole is arranged in same edges with three alternately-distributed first honeycomb holes adjacent to the firsthoneycomb hole and three alternately-distributed second honeycomb holes adjacent to the first honeycomb hole. The internal structure of the bioreactor is formed by vertically stacking honeycomb carrier plates and air-permeable hollow fiber membrane tubes layer by layer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a honeycomb carrier plate for culturing liver cells in vitro and a bioreactor for artificial liver. Background technique [0002] Artificial liver support system is an effective treatment method for patients with liver failure, currently divided into two categories: non-biological artificial liver and biological artificial liver. Non-biological artificial liver is the type of artificial liver widely used in clinical practice. It can temporarily compensate the detoxification effect of the liver, but it cannot completely replace the synthesis, metabolism and biotransformation functions of the liver. Based on in vitro liver cell source and bioreactor The bioartificial liver is theoretically closer to the physiological function of the human liver, and is currently the main international development direction. Bioreactor is the core device of bioartificial liver treat...

AI Technical Summary

Problems solved by technology

[0009] The embodiment of the present application provides a honeycomb carrier plate for in vitro culture of hepatocytes and a bioreactor for artificial liver, which are used to solve the problems of insufficient space for the growth of hepatocytes in vitro in a bioartificial liver and low material exchange efficiency

Images