Method for determining diethylstilbestrol by adopting up-conversion luminescence immunochromatography

Abstract

The invention discloses a method for determining diethylstilbestrol by adopting up-conversion luminescence immunochromatography. The method is characterized in that according to the research, a solvothermal method is adopted to synthesize up-conversion nanoparticles (UCNPs)-NaYF4: Yb, Er, surface functionalization modification is carried out, the nanoparticles are coupled with a diethylstilbestrol(DES) monoclonal antibody to prepare a fluorescent probe, a nitrocellulose film is respectively sprayed on a bovine serum albumin-diethylstilbestrol (BSA-DES) conjugate and a goat anti-mouse secondary antibody to form a test strip detection line (T line) and a quality control line (C line), and an up-conversion luminescence immunochromatographic test strip is established for rapid quantitative detection; a method of DES is adopted; the experimental result shows that the DES linear quantitative detection range of the test strip is 25 to 10000 ng/mL (Y is equal to 0.43927 x-0.57647, and R2 is equal to 0.996), the detection limit is 20.84 ng/mL, the detection time of a single sample is 15 min, intra-batch and inter-batch variable coefficients are both smaller than 10%, the specificity recognition capacity is high, an average value of RSD detection values is about 15% after the kit is stored at 37 DEG C for 7 days; standard recovery experiments show that the average recovery rate is 90.1%-115.2%, relative standard deviation is smaller than 5%, and the method has good consistency with high performance liquid chromatography.

Description

technical field [0001] The invention belongs to the field of biological testing. It specifically relates to a method for determining diethylstilbestrol. Background technique [0002] Dethylstilbestrol (DES), as a synthetic estrogen drug, is widely used in the prevention and treatment of gynecological diseases, and it also has the functions of promoting animal growth and improving feed conversion rate. DES can enter the human body through the food chain, leading to serious consequences such as abnormal secretion of hormones, even breast cancer and embryonic deformities. Therefore, many countries have explicitly banned the use of DES in animal breeding. The DES detection method stipulated in my country's national standards is liquid chromatography-tandem mass spectrometry. Currently, the methods used for DES detection mainly include enzyme-linked immunoassay, high performance liquid chromatography, liquid chromatography-mass spectrometry, electrochemical method, radioimmunoas...

AI Technical Summary

Problems solved by technology

At present, the research on up-conversion luminescence immunochromatography technology has gradually attracted attention. Yu Ran et al. used carboxyl-modified up-conversion particles in immunochromatography test paper to detect deoxynivalenol, but the antibody labeling is complicated and the detection range is narrow.

Images