A saline-alkali-resistant molecular marker c72 of Portunus trituberculatus and its application

Abstract

The invention provides a saline-alkali-resistant molecular marker C72 of Portunus trituberculatus and its application. The nucleotide sequence of the molecular marker C72 is shown in SEQ ID No.1, and the nucleotide sequence of the primer pair for detecting the molecular marker C72 is shown in SEQ ID No.2 and SEQ ID No.3 . The molecular marker C72 is a SNP marker, and its saline-alkali tolerance genotype is CT genotype. The molecular marker C72 provided by the present invention can not be limited by the growth stage of Portunus trituberculatus, and can obviously speed up the breeding process of Portunus trituberculatus and quickly breed crab species with excellent characteristics of tolerance to salt and alkali, and use the molecular marker C72 The detection of the salt-alkali tolerance of Portunus trituberculatus is accurate, reliable and easy to operate, which is beneficial to the healthy breeding and development of Portunus trituberculatus, so it has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of DNA molecular markers of aquatic animals, and in particular relates to a saline-alkali-resistant molecular marker C72 of Portunus trituberculatus and its application. Background technique [0002] Swimming crab (Portunus trituberculatus) belongs to Crustacea, Decapod order, Portunidae, commonly known as swimming crab, is an important large-scale marine economic crab in my country. Swimming crab meat is delicious and nutritious, and is deeply loved by consumers. Saline-alkaline waters are a worldwide low-yield water resource. In China alone, there are 45.87 million hm2 of saline-alkaline waters. Due to the characteristics of high salinity, high alkalinity, high pH and complex ion composition, conventional aquatic animals cannot Among them, normal survival and reproduction hinder the development and utilization of this type of water resources to a large extent. The physiology and biochemistry of Portunus ...

AI Technical Summary

Problems solved by technology

Saline-alkaline waters are a worldwide low-yield water resource. In China alone, there are 45.87 million hm2 of saline-alkaline waters. Due to the characteristics of high salinity, high alkalinity, high pH and complex ion composition, conventional aquatic animals cannot Among them, normal survival and reproduction hinder the development and utilization of such water resources to a large extent.

The physiology and biochemistry of Portunus trituberculatus will be affected to a certain extent under the stimulation of high salinity, and high salinity will reduce the feeding rate, metamorphosis rate and survival rate of young crabs

However, the trait of salt-alkaline tolerance has obvious low heritability characteristics, and the genetic progress through traditional breeding methods is slow. It is urgent to use advanced molecular marker-assisted breeding technology to speed up the breeding process, and the identification and innovative application of salt-alkali tolerance molecular markers It is the necessary prerequisite and way to carry out molecular marker assisted breeding

[0003] At present, there are few studies on the development of molecular markers for salt-alkali tolerance in Portunus trituberculatus, and there is a lack of markers that can be applied to molecular marker-assisted breeding in the industry

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