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Composite microbial agent for strengthening anaerobic degradation of propionic acid and construction method for composite microbial agent

A technology of compound bacterial agent and oxidative bacteria, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of limited physiological and ecological functions and deficiencies of methanogenic bacteria

Active Publication Date: 2020-06-19
江门市环木工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above problems, the purpose of the present invention is to provide a method for strengthening the degradation of propionic acid by constructing fatty acid oxidizing bacteria and electrogenic bacteria mutual symbiont complex bacterial agent, which solves the dependence of propionic acid mutual degradation in traditional anaerobic systems. Methanogens are thus limited by the lack of physiological and ecological functions of methanogens

Method used

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  • Composite microbial agent for strengthening anaerobic degradation of propionic acid and construction method for composite microbial agent
  • Composite microbial agent for strengthening anaerobic degradation of propionic acid and construction method for composite microbial agent
  • Composite microbial agent for strengthening anaerobic degradation of propionic acid and construction method for composite microbial agent

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Embodiment 1

[0041] This embodiment provides a composite bacterial agent that strengthens the anaerobic degradation of propionic acid and its construction method. The composite bacterial agent includes fatty acid oxidizing bacteria Syntrophobacterfumaroxidans and electrogenic bacteria Geobactorsulfurreducens (hereinafter referred to as: G.sulfurreducen); the composite bacterial agent The build process of the agent is as follows:

[0042] (1) Activation and enrichment of G.sulfurreducen

[0043] G.sulfurreducens was purchased from the American Type Culture Collection (ATCC, number 51573). The components of the G.sulfurreducens medium used in this example mainly refer to the medium components recommended by ATCC, and are improved according to the actual situation of this experiment and relevant literature. The components are shown in Tables 1-3.

[0044] Table 1 Complete medium of G.sulfurreducens

[0045] Element content Element content sodium acetate 1.64g / L NH 4...

Embodiment 2

[0073] According to the system of the present invention, the effects of different concentrations of propionic acid, different concentrations of ammonia nitrogen and different pH on the metabolic growth of mutual symbionts were studied respectively.

[0074] (1) To study the effects of different propionic acid concentrations (2000-4500mg / L) on the metabolic growth of mutual symbionts, the operation period is 19 days, and three groups are operated at the same time.

[0075] (2) To study the effects of different ammonia nitrogen concentrations (500-3000mg / L) on the metabolic growth of mutual symbionts. Propionic acid was 1500mg / L, and the operation period was 19 days. Three groups were operated at the same time.

[0076] (3) To study the effects of different pH (5.5-8.0) on the metabolic growth of mutual symbionts, propionic acid 1500mg / L, the operation cycle is 19 days, and three groups are operated at the same time.

[0077] In order to reduce the risk of bacterial contaminatio...

Embodiment 3

[0082] Further study the application effect of the composite bacterial agent of the present invention in actual wastewater. The composite bacterial agent constructed under the optimum conditions in Example 2 was added to the sludge dehydration liquid anaerobic fermentation system and regulated according to the above-mentioned optimal factor range, the inoculum ratio of the composite bacterial agent was 20.5±5.5g VSS / L sewage . Measure the VFAs value before and after water out, the results are as follows Figure 5 .

[0083] Depend on Figure 5 The results showed that the effluent VFAs decreased from 1013mgHAc / L to 245mgHAc / L, a decrease of 75.8%, among which propionic acid decreased by 83.9%, which was a significant decrease. It shows that the composite microbial agent of the present invention is better applied in the process of wastewater treatment.

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Abstract

The invention discloses a composite microbial agent for strengthening anaerobic degradation of propionic acid and a construction method for the composite microbial agent. The composite microbial agentcomprises Syntrophobacterfumaroxidans and Geobactorsulfurreducens. According to the composite microbial agent, a syntrophism symbiosis system for strengthening the degradation of the propionic acid is constructed through activating the Syntrophobacterfumaroxidans and the Geobactorsulfureducens, taking an anaerobic bottle inserted electrode as an experimentation mode, and adding an activating bacterial solution into an anode chamber; the composite microbial agent can be applied to a sewage treatment process; and the method has the advantages of simple structure, flexibility in operation, rapidness in start-up, stability in operation, no need of aeration, low operating cost and the like, and optimal conditions for degradation of the propionic acid can be regulated and controlled through a pH value, ammonia nitrogen and an initial propionic acid concentration value.

Description

technical field [0001] The invention belongs to the technical field of anaerobic fermentation, and relates to a microbial battery, in particular to a composite bacterial agent for strengthening the anaerobic degradation of propionic acid and a construction method thereof. Background technique [0002] Anaerobic biotechnology is widely used to treat high-concentration organic wastewater and has achieved good practical results due to its ability to simultaneously reduce organic matter in wastewater and increase productivity. The basic principle is to realize the gradual degradation of organic matter and the production of methane through the synergistic effect of hydrolytic fermentation bacteria, acetogenic bacteria and methanogenic bacteria. One of the main challenges to the efficient and stable operation of conventional anaerobic processes is the acid inhibition problem caused by the accumulation of volatile fatty acids (Volatile Fatty Acids, VFAs) in the system. During the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/28C02F3/00C12R1/01C02F101/34
CPCC02F3/005C02F3/28C02F3/34C02F2101/34C12N1/20
Inventor 汪涛匡彬朱葛夫郏建波刘敏超李玉瑛李冰杨涛张梦辰徐晓龙
Owner 江门市环木工程技术有限公司
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