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A strain of Microbacterium aureus with high efficiency for purifying sewage and its application
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A technology of Microbacterium aureus and strains, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc.
Active Publication Date: 2022-05-27
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However, there is no report on the isolation of Microbacterium aureus from black and odorous water in Beijing for the treatment of black and odorous water.
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[0037] Two representative sites were selected from the Heiwu reach of Wenyu River in Changping District, Beijing (116.221176 E, 40.168619 N), and about 20 g of surface sediment was mixed and brought back to the laboratory for storage at 4°C.
[0040] Weigh 10g of bottom mud and 90mL of sterile physiological saline and fully mix it to make a soil suspension; draw 1mL of soil suspension (100 gradient) and add it to 9mL of sterile physiological saline, and mix well to obtain 10. -1 Diluent; and so on, making a total of 100 to 10 -7 A total of 8 serial dilutions.
[0041] (2) Bacterial isolation and identification
[0042] Prepare LB medium plate, pipette 200 μL of each gradient dilution solution onto the plate respectively, spread evenly, make two repetitions of each gradient, set aseptic control...
[0051] (1) Research on the growth curve of Microbacterium aureus under different temperature conditions
[0052] 14 bacterial culture tubes were taken, each tube was filled with 5 mL of LB liquid medium, sterilized, and then 2% Microbacillus aureus solution was added, and the culture was shaken at 20°C and 37°C, respectively. Samples were taken at 0h, 2h, 4h, 6h, 8h, 10h, and 12h, respectively, and stored at 4°C, and the absorbance value OD of the bacterial solution was measured. 600 .
[0053] The growth curves of Microbacterium aureus at different temperatures are as follows: figure 2 shown. Depend on figure 2 It can be seen that in the initial stage, the lower temperatur...
Embodiment 3
[0061] Research on the ability to purify synthetic sewage
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Abstract
The invention provides a microbacterium aureus strain for efficiently purifying sewage and its application, belonging to the technical field of functional microorganisms. The preservation number of Chryseomicrobium imtechense strain YG-01 is CGMCC No.19535. The strain YG‑01 is not sensitive to strong alkali and high salt conditions, and can grow normally under the conditions of pH value 7.0-10 and salinity 0.5‰-10‰. The present invention also provides the application of the bacterial strain YG‑01 in sewage purification. Compared with the previous studies on the use of bacteria for sewage purification, the strain YG‑01 has a higher COD removal rate for sewage under suitable conditions, and is not sensitive to alkaline water, so it has the potential to be used as a strain for purifying polluted water.
Description
technical field [0001] The invention belongs to the technical field of functional microorganisms, and in particular relates to a strain of Microbacterium aureus capable of efficiently purifying sewage and its application. Background technique [0002] The treatment of black and odorous water bodies is one of the hot topics in the field of environmental management in my country. According to the information released by the Beijing Water Affairs Bureau, there are also many black and odorous water bodies in Beijing that need to be treated (Beijing Water Affairs Bureau website). Bacterial microorganisms play an important role in the purification and restoration of black and odorous water bodies (Zhang Zongyang, 2010; Huang Feifei, 2012; Cheng soldiers, 2012). Some scholars have isolated microorganisms such as bacteria from black and odorous water bodies, and verified that the isolated strains have a good effect in the purification of black and odorous water bodies or sewage. F...
Claims
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Application Information
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