Method for simultaneously determining content of cystine and methionine in artificial tiger bone powder

Abstract

The invention relates to a method for simultaneously determining the content of cystine and methionine in artificial tiger bone powder. The method comprises the following steps: (1) taking an artificial tiger bone powder sample, adding a performic acid solution to oxidize, adding hydrobromic acid to terminate oxidation, evaporating to dryness, adding hydrochloric acid to hydrolyze, evaporating todryness, dissolving with a derivative buffer solution, and deriving to obtain a test solution; (2) taking sulfoalanine and methionine sulfone reference substances, and dissolving the sulfoalanine andmethionine sulfone reference substances with a derivative buffer solution to derive to obtain a mixed reference substance solution; (3) carrying out chromatographic detection on the test solution andthe reference solution, wherein a chromatographic column takes cyano bonded silica gel as a filler; the column temperature is 20 DEG C; a mobile phase: A is a sodium acetate buffer solution; B is methanol-acetonitrile-water, and gradient elution is carried out; and (4) calculating to obtain the contents of sulfoalanine and methionine sulfone in the artificial tiger bone powder according to a detection result, and further calculating to obtain the content of cystine and methionine sulfone in the artificial tiger bone powder. The detection method is accurate, reliable, simple, convenient and rapid.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and relates to a method for simultaneously measuring the content of cystine and methionine in artificial tiger bone meal. Background technique [0002] Artificial tiger bone powder is a bone-building drug and a new generation of tiger bone substitute. It has the functions of strengthening muscles and bones, dispelling rheumatism, and relieving pain. It is mainly used clinically for osteoporosis, fractures, rheumatoid arthritis, chronic low back pain, etc. Treatment of bone and joint diseases. The composition of artificial tiger bone powder is basically the same as that of natural tiger bone. Studies have shown that its pharmacology, efficacy and safety indicators are not significantly different from those of natural tiger bone. The quality control standard of bone powder, this quality control method only controls the content of 16 kinds of amino acids needed by the...

AI Technical Summary

Problems solved by technology

[0004] Although, in the prior art, there have been some detection methods for cystine and methionine, most of the existing detection methods are for detecting items with high cystine and methionine content such as milk powder and feed The content of cystine and methionine, its extraction and detection are relatively simple (less interference factors)

[0005] The artificial tiger bone meal is composed of animal bones and has a hard texture. It is difficult to extract cystine and methionine, and their content is small and difficult to detect (many interference factors)

The chromatographic column used in the existing detection methods of methionine and cystine is ordinary C 18 column, the column temperature is 30°C, 35°C and 40°C, the mobile phase is sodium acetate buffer solution (weigh 3.56g anhydrous sodium acetate, dissolve it in 1000ml water, add a volume fraction of 0.1% triethylamine, use glacial acetic acid Adjust the pH to 6.4) as mobile phase A, and use acetonitrile-water (50:50) as mobile phase B to carry out different gradient experiments. The chromatographic conditions cannot accurately quantify methionine and cystine in artificial tiger bone powder (Methionine and cystine chromatographic peaks and other impurity peaks in the chromatogram cannot be effectively separated); therefore, existing assay methods cannot be directly used for cystine and methionine in artificial tiger bone meal detection

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