Polysaccharide fermentation composition capable of resisting cancer, virus and inflammation, promoting osteoblast proliferation and promoting intestinal stem cell proliferation and preparation method
A technology for fermenting compositions and polysaccharides, applied in biochemical equipment and methods, microorganism-based methods, antiviral agents, etc., can solve problems such as difficult absorption, loss of appetite, residual toxicity of solvents, etc.
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Embodiment 1
[0047] Embodiment 1: the preparation method of polysaccharide fermentation composition
[0048] Using one or more polysaccharide-containing vegetables, mushrooms, and algae as raw materials (lotus root, yam, aloe vera, shiitake mushrooms, fungus, white fungus, sea fungus, cauliflower, Ulva, green silk algae, sea grapes, and kudzu algae), Through special fermentation technology, the polysaccharide molecules (β-glucan, α-glucan) are converted and divided into small molecular saccharide compounds with a molecular weight of about 300 Daltons. Its structure is different from ordinary polysaccharide molecules and is in an organic form. After fermentation, the excess glucose structure on the branch chain is metabolized by the bacteria to remove excess glucose molecules, which can make the sugar molecules have affinity and become a polysaccharide fermentation composition that can be coated, and has strong anti-cancer properties, anti-virus properties, Anti-inflammatory properties, pro...
Embodiment 2
[0060] Example 2: Polysaccharide fermentation composition and polysaccharide water extract component comparison
[0061] The raw materials of the present invention are 12 planting plants including lotus root, yam, aloe, shiitake mushroom, fungus, white fungus, sea fungus, cauliflower, Ulva, green silk algae, sea grape, and kudzu. Mixed in equal proportions, each raw material is fermented independently, with pineapple, papaya, and apple as the substrate, and fermented for 90 to 180 days, a total of 2 strains (yeast: Saccharomyces cerevisiae; lactic acid bacteria: Lactobacillus plantarum) are fermented and produced. Mixing in equal proportions, extracting the sugar compound with ethanol, and freeze-drying the precipitated sugar compound.
[0062] The following experiments are divided into 2 groups:
[0063] (1) Polysaccharide fermented liquid
[0064] (2) Polysaccharide water extract (Polysacchride extract): A total of 12 plants of lotus root, yam, aloe vera, shiitake mushroom...
Embodiment 3
[0070] Embodiment 3: the antiviral test of polysaccharide fermentation composition
[0071] Divide the following experiments into 4 groups:
[0072] (1) Polysaccharide fermentation composition (Polysacchride structure extract): The test was carried out on samples of sugar structures below 300 Daltons after extraction.
[0073] (2) Water-extracted polysaccharides (Polysacchride extract): the test was carried out on samples of sugars below 300 Daltons after extraction.
[0074] (3) Virus attack group (Virus): no samples were added.
[0075] (4) Non-challenge (Control): no samples were added.
[0076] Divided into two ways to test: 1. the protective effect on cells: after veto cells (Vero) were co-cultured with each sample for 4 hours, enterovirus 71 virus was added and cultivated for 24 hours; 2. the resistance to viruses: virus and Four hours after sample mixing, cells were added and incubated for 24 hours. Subsequently, the cell viability was tested by MTT assay. The conc...
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