Polysaccharide fermentation composition capable of resisting cancer, virus and inflammation, promoting osteoblast proliferation and promoting intestinal stem cell proliferation and preparation method

A technology for fermenting compositions and polysaccharides, applied in biochemical equipment and methods, microorganism-based methods, antiviral agents, etc., can solve problems such as difficult absorption, loss of appetite, residual toxicity of solvents, etc.

Inactive Publication Date: 2020-06-23
BIOZYME BIOBTECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to current research, it has been confirmed that mushrooms and algae are rich in polysaccharides, which have many anti-cancer, anti-virus, and anti-inflammatory effects. However, the molecular weight of polysaccharides obtained by extraction is about 6,000 Daltons. The ingredients are functional, and their molecular weight must be greater than 6,000 Daltons or less than 500 Daltons. However, for cancer patients, the side effects of chemotherapy will cause side effects such as loss of appetite and poor gastrointestinal absorption rate. If polysaccharides are in the form of macromolecules As a complementary food, whether it can be absorbed or intestinally utilized by patients is still questionable
[0006] Moreover, the extraction of polysaccharides known to date is mainly based on water extraction or organic solvent extraction. Its efficacy is naturally possessed and cannot effectively improve the efficacy. Its molecular weight is about 6,000 Daltons or more, and there is a problem of poor absorption rate for patients. , the action of hydrolytic enzymes is needed before absorption. If the molecular weight is reduced to less than 500 Daltons before ingestion, the shortcoming of difficult absorption can be solved
[0007] And in another study on algae, polysaccharides and polyphenols in algae have anti-oxidation, anti-cancer, anti-coagulation, anti-inflammation, anti-virus, anti-hyperlipidemia and other effects. In order to obtain these active substances, the industry usually uses Extraction methods such as hot water, solvent, acid hydrolysis, and enzyme hydrolysis, but these extraction methods are usually accompanied by some limiting factors, including: high cost, complicated process, and residual toxicity of solvents

Method used

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  • Polysaccharide fermentation composition capable of resisting cancer, virus and inflammation, promoting osteoblast proliferation and promoting intestinal stem cell proliferation and preparation method
  • Polysaccharide fermentation composition capable of resisting cancer, virus and inflammation, promoting osteoblast proliferation and promoting intestinal stem cell proliferation and preparation method
  • Polysaccharide fermentation composition capable of resisting cancer, virus and inflammation, promoting osteoblast proliferation and promoting intestinal stem cell proliferation and preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: the preparation method of polysaccharide fermentation composition

[0048] Using one or more polysaccharide-containing vegetables, mushrooms, and algae as raw materials (lotus root, yam, aloe vera, shiitake mushrooms, fungus, white fungus, sea fungus, cauliflower, Ulva, green silk algae, sea grapes, and kudzu algae), Through special fermentation technology, the polysaccharide molecules (β-glucan, α-glucan) are converted and divided into small molecular saccharide compounds with a molecular weight of about 300 Daltons. Its structure is different from ordinary polysaccharide molecules and is in an organic form. After fermentation, the excess glucose structure on the branch chain is metabolized by the bacteria to remove excess glucose molecules, which can make the sugar molecules have affinity and become a polysaccharide fermentation composition that can be coated, and has strong anti-cancer properties, anti-virus properties, Anti-inflammatory properties, pro...

Embodiment 2

[0060] Example 2: Polysaccharide fermentation composition and polysaccharide water extract component comparison

[0061] The raw materials of the present invention are 12 planting plants including lotus root, yam, aloe, shiitake mushroom, fungus, white fungus, sea fungus, cauliflower, Ulva, green silk algae, sea grape, and kudzu. Mixed in equal proportions, each raw material is fermented independently, with pineapple, papaya, and apple as the substrate, and fermented for 90 to 180 days, a total of 2 strains (yeast: Saccharomyces cerevisiae; lactic acid bacteria: Lactobacillus plantarum) are fermented and produced. Mixing in equal proportions, extracting the sugar compound with ethanol, and freeze-drying the precipitated sugar compound.

[0062] The following experiments are divided into 2 groups:

[0063] (1) Polysaccharide fermented liquid

[0064] (2) Polysaccharide water extract (Polysacchride extract): A total of 12 plants of lotus root, yam, aloe vera, shiitake mushroom...

Embodiment 3

[0070] Embodiment 3: the antiviral test of polysaccharide fermentation composition

[0071] Divide the following experiments into 4 groups:

[0072] (1) Polysaccharide fermentation composition (Polysacchride structure extract): The test was carried out on samples of sugar structures below 300 Daltons after extraction.

[0073] (2) Water-extracted polysaccharides (Polysacchride extract): the test was carried out on samples of sugars below 300 Daltons after extraction.

[0074] (3) Virus attack group (Virus): no samples were added.

[0075] (4) Non-challenge (Control): no samples were added.

[0076] Divided into two ways to test: 1. the protective effect on cells: after veto cells (Vero) were co-cultured with each sample for 4 hours, enterovirus 71 virus was added and cultivated for 24 hours; 2. the resistance to viruses: virus and Four hours after sample mixing, cells were added and incubated for 24 hours. Subsequently, the cell viability was tested by MTT assay. The conc...

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Abstract

The composition provided by the invention contains polysaccharide vegetables, mushrooms and algae as raw material substances, polysaccharide molecules (beta glucan and alpha glucan) are converted andsegmented into small molecular form saccharides through a special fermentation preparation method, and the composition is a polysaccharide fermentation composition with the molecular weight of about 300 Daltons, has a structure different from that of common polysaccharide molecules, is in an organic form, and can be used for preparing polysaccharide products. After the composition is fermented, redundant glucose structures on branched chains are metabolized by thalli, redundant glucose molecules are removed, the sugar molecules can have affinity, and the polysaccharide fermentation compositionwith the coating material has the effects of resisting cancers, viruses and inflammation, promoting osteoblast proliferation and promoting intestinal stem cell proliferation.

Description

technical field [0001] The invention provides a composition containing polysaccharide vegetables, mushrooms, and algae (such as: lotus root, yam, aloe, mushroom, fungus, tremella, sea fungus, cauliflower, Ulva, green hair algae, sea grapes, kudzu rice algae) As a raw material, polysaccharide molecules (β-glucan, α-glucan) are converted and split into small molecule sugars through a special fermentation preparation method, which is a polysaccharide fermentation composition with a molecular weight of about 300 Daltons. Different from general polysaccharide molecules, it is an organic form. After fermentation, the excess glucose structure on the branch chain is metabolized by the bacteria to remove excess glucose molecules, which can make the sugar molecules have affinity and become a coating material, which has anti-cancer and anti-virus properties. , Anti-inflammation, promote osteoblast proliferation, and promote the effect of intestinal stem cell proliferation. Background t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04A61K31/715A61P35/00A61P31/14A61P29/00A61P19/08A61P1/00C12R1/25C12R1/23C12R1/84C12R1/865C12R1/645C12R1/01
CPCC12P19/04A61K31/715A61P35/00A61P31/14A61P29/00A61P19/08A61P1/00
Inventor 黄柽赖怡君赖柏儒
Owner BIOZYME BIOBTECH CORP
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