A Method for Acquiring Microscopic Motion Information Under Shear Field Using Fluorescence Wide-field Imaging

Abstract

The invention discloses a method for obtaining microscopic motion information under a shear field by using the trajectory light intensity distribution of fluorescence wide-field imaging. The method is a measurement and analysis method for obtaining motion information at the single-molecule scale through the light intensity distribution of the fluorescent particle motion track. The method uses the polymer microrheological spectrometer independently developed by the research group, and combines macroscopic shear application and rheological measurement technology with microscopic fluorescence wide-field microscopic imaging technology. This method has single-molecule level sensitivity and resolution rate; and can analyze the light intensity distribution characteristics through software, and successfully obtain the particle motion information and its change with the external shear field from the microscopic level. This method will also be widely used in the field of basic research on polymer physics under complex flow fields, and to obtain microscopic physical images and motion information.

Description

technical field [0001] The invention belongs to the field of polymer physics, and relates to a method for obtaining microscopic motion information under a shear field by using fluorescence wide-field imaging. Background technique [0002] In the field of basic research in polymer physics, the characteristics of shear flow field and the rheological properties of molecules, particles, polymer chains such as multi-charged soft materials under shear field and their correlation with microscopic motion and structure have always been important. It is a hot research topic in academia and industry, but it is also a difficult topic. Research in this area not only helps to better understand the microscopic physical processes under the flow field, but also has important guiding significance for the research in the biological field and the design and manufacture of high-end microfluidic devices. [0003] The measurement under the traditional shear flow field usually adopts the contact m...

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Problems solved by technology

[0004] However, because the fluorescence correlation spectroscopy method cannot directly obtain real microscopic images, it has a fitting model dependence: on the one hand, in a low-velocity flow field, the flow characteristic time caused by steady shear is different from the characteristic time of the fluorescent probe’s own diffusion When the model is similar, it is difficult for the model to obtain the motion information under the low-speed steady-state shear field; on the other hand, it is difficult to obtain the microscopic motion state and information of the more common complex shear modes such as oscillating shear through model fitting

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