Subject-specific tumor inhibiting cells and the use thereof
A subject and cell technology, applied in the direction of antineoplastic drugs, animal cells, vertebrate cells, etc., can solve problems such as transient effects and interference with immune tolerance
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[0205] The invention is further defined in the following examples. It should be understood, that these Examples, while indicating preferred embodiments of the invention, are given by way of illustration only. From the above discussion and these Examples, one skilled in the art can ascertain the essential characteristics of this invention, and without departing from the spirit and scope of the invention, can make various changes and modifications of the invention to adapt it to various usages and conditions.
[0206] Materials and methods
[0207] Source of T cells:
[0208] Human primary T cells were purified from the peripheral blood of healthy donors. Human TILs were isolated from fresh breast tumor samples according to approved procedures and protocols.
[0209] Isolation of T cells from blood:
[0210] From each healthy donor recruited at the clinical facility of the Institut de Recherche Clinique de Montreal (IRCM), approximately 40 mL of venous blood was collected...
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