Reagent composition and method for preserving integrity of nucleic acid in human saliva

A technology of composition and integrity, applied in the direction of biochemical equipment and methods, microbiological determination/inspection, etc., can solve problems such as inability to preserve saliva DNA for a long time

Active Publication Date: 2020-07-07
HANGZHOU KMB BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the saliva preservation solutions currently on the market cannot guarantee the purity and integrity of saliva DNA, and the better saliva preservation solutions on the market cannot preserve saliva DNA for a long time

Method used

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  • Reagent composition and method for preserving integrity of nucleic acid in human saliva
  • Reagent composition and method for preserving integrity of nucleic acid in human saliva
  • Reagent composition and method for preserving integrity of nucleic acid in human saliva

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The composition for preserving human saliva, its components and content are: Tris-HCl 0.2mol / L at pH 8.0; urea 0.45mol / L; SDS 0.6%; CDTA 3.6mmol / L; ethanol 40%; Propyl formate 2mg / mL; Diazolidinyl imidazole urea 2mg / mL; Sodium acetate 0.67mol / L; Sodium ascorbate 0.05mol / L; Sucrose 4%; Proteinase K 10ug / mL; System pH 8.0.

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PUM

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Abstract

The present invention provides a reagent composition and method for preserving the integrity of nucleic acid in human saliva and an application of the kit composition. The reagent composition comprises urea, sodium lauryl sulfate, trans-1, CDTA, Tris-HCl, sodium ascorbate, propyl p-hydroxybenzoate, diazolidinyl urea, ethanol, sucrose and protease K. The reagent composition and the human saliva aremixed uniformly and then are stored at the normal temperature. The reagent composition and the preservation method can inhibit the nuclease activity and protect nuclease from being contaminated withexternal microorganisms or being oxidized, thereby ensuring the purity and integrity of genomic DNA; and the preservation time is long, the applicable storage temperature range is wide, and the stability of a saliva sample in a process of transportation among acquisition places can be ensured.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically to a reagent composition and method for preserving the integrity of nucleic acid in human saliva. Background technique [0002] At present, molecular biology detection technology has been increasingly used in many fields such as forensic medicine, military affairs, human medicine and scientific research. For example, in terms of human health, by extracting DNA from the blood, other body fluids or cells of the tester, and performing nucleic acid sequence amplification, and then detecting the target sequence or susceptibility loci to collect relevant gene information, the known information The interpretation enables people to understand their genetic information, predict the risk of physical illness, thereby improving their living environment and habits, and avoiding or delaying the occurrence of diseases. Another example is in the field of forensic identification, the identity of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2527/125
Inventor 林源吉丁佳女叶俊波
Owner HANGZHOU KMB BIOTECH
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