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Construction and detection method of calibration model for determining content of effective components in compound liquorice tablet

A technology of compound licorice tablets and active ingredients, which is applied in measuring devices, analyzing materials, and analyzing materials through optical means, and can solve problems such as weak absorption intensity, low spectral signal-to-noise ratio, and difficult quantitative analysis of NIR spectra

Pending Publication Date: 2020-07-07
GUANGZHOU BAIYUSN GUANGHUA PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the active ingredients of the compound medicine prepared with licorice are more complex than those of licorice. When the active ingredients of the compound medicine are detected by infrared spectroscopy, the absorption intensity of the characteristic spectral region of the active ingredients is weak, and the signal-to-noise ratio of the spectrum is low. The double frequency and combined spectrum bands overlap severely, making quantitative analysis of NIR spectra very difficult
Based on this problem, although the use of near-infrared analysis in the detection of medicinal materials has been disclosed, there is no literature report on the use of near-infrared analysis to quickly determine the content of active ingredients (such as glycyrrhizic acid) in the compound preparation of compound licorice tablets

Method used

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  • Construction and detection method of calibration model for determining content of effective components in compound liquorice tablet
  • Construction and detection method of calibration model for determining content of effective components in compound liquorice tablet
  • Construction and detection method of calibration model for determining content of effective components in compound liquorice tablet

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Embodiment 1

[0032] The assay method of glycyrrhizic acid content in embodiment 1, compound licorice tablet

[0033] The present embodiment provides a kind of assay method of glycyrrhizic acid content in compound licorice tablet, comprises the steps:

[0034] (1) Calibration set and raw spectral data acquisition

[0035] A total of 80 batches were collected from compound licorice tablets, 60 batches were selected as the calibration set, and 20 batches were selected as the verification set. The samples were crushed through a 60-mesh sieve and dried in an oven at 70°C for 3 hours.

[0036] Utilize the near-infrared spectrometer to collect the original spectral data of the calibration set to obtain the original near-infrared spectral data of Compound Licorice Tablets, see figure 1 .

[0037] Near-infrared spectrometer: Antaris II FT-NIR Analyzer Thermo Fisher Scientific Corporation, the United States, light source; tungsten-halogen lamp, detector: InGaAs, integrating sphere diffuse reflec...

Embodiment 2、 Embodiment 1

[0066] The applicability verification of constructing correction model in the method of embodiment 2, embodiment 1

[0067] This embodiment is to verify the applicability of the calibration model constructed in embodiment 1. The difference between this embodiment and embodiment 1 is mainly that the object of content determination in step (5) is licorice extract. Specifically, step (5) of this embodiment includes:

[0068] Utilize the NIR instrument to collect the original spectral data of the licorice extract sample, input it into the TQ quantitative analysis software that has been imported into the calibration model, and obtain the content of glycyrrhizic acid in the licorice extract through software calculation, that is, the predicted value; Octadecylsilane bonded silica gel is used as filler, acetonitrile is used as mobile phase A, and 0.05% phosphoric acid solution is used as mobile phase B for gradient elution. The content determination method under the licorice extrac...

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Abstract

The invention provides a construction and detection method of a calibration model for determining the content of effective components in a compound liquorice tablet. The construction method comprisesthe following steps: taking a plurality of compound liquorice tablet samples as a calibration set, and performing near-infrared light scanning to obtain original spectral data of the calibration set;carrying out spectrum preprocessing on the original spectrum data of the calibration set, and selecting a modeling waveband of the effective component to obtain a content characteristic spectrum of the effective component; respectively determining the effective components of a plurality of compound liquorice tablet samples contained in the calibration set by adopting a high performance liquid chromatography to obtain the true content value of the effective components; and associating the obtained content characteristic spectrum with the content true value by using a partial least square method, and establishing a content correction model of the effective component. When the content correction model is used for detecting the content of glycyrrhizic acid in the compound liquorice tablet, background noise and physical factor dryness can be effectively eliminated, the prediction capability is good, and the detection result is accurate; meanwhile, the content correction model also has verystrong applicability.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a method for constructing a correction model for determining the content of active ingredients in compound licorice tablets and a detection method. Background technique [0002] Compound licorice tablets were introduced from the United States more than 50 years ago, and the original prescription came from Brown Mixtura invented by Dr. John Brown (1735-1788). In 1963, Qinghai Pharmaceutical Factory deleted the first registration of compound licorice tablets in Brown's original prescription of antimony potassium tartrate, but the compound licorice oral liquid is still used. The formula of Compound Licorice Tablets has undergone several changes, and finally each tablet contains 112.5 mg of licorice extract powder, 4 mg of opium powder / poppy fruit extract, 2 mg of camphor, 2 mg of star anise oil, and 2 mg of sodium benzoate. Its curative ef...

Claims

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Application Information

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IPC IPC(8): G01N21/359G01N21/3563
CPCG01N21/359G01N21/3563G01N21/274
Inventor 杨建锐胡卫林何敏儿霍宝瑜胡艳云梁燕明
Owner GUANGZHOU BAIYUSN GUANGHUA PHARMA
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