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Preparation method and electrode of composite material modified electrode for detecting glucose

A technology for modifying electrodes and composite materials, which is applied in the preparation of electrochemical biosensors. It can solve the problems of unsatisfactory electrode selectivity, low detection limit, weak glucose response signal, etc., and achieve excellent catalytic performance and detection effect and low cost. , the effect of fast detection speed

Active Publication Date: 2021-06-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] If the working electrode in the electrochemical analysis method is used directly in the form of a bare electrode without modification, the response signal of glucose is weak, the detection limit is low, and the selectivity of the electrode cannot meet the requirements of application in actual blood samples

Method used

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  • Preparation method and electrode of composite material modified electrode for detecting glucose
  • Preparation method and electrode of composite material modified electrode for detecting glucose
  • Preparation method and electrode of composite material modified electrode for detecting glucose

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The polymethylene blue composite modified electrode of the present invention is prepared by the following method:

[0028] (1) Mix 0.5 μm and 0.05 μm alumina powder with deionized water and volume ratio of 1:1, pour the dispersion formed by 0.5 μm alumina on the rough suede, and glassy carbon electrode After polishing, pour the dispersion formed by 0.05μm alumina on the finer suede, and continue to polish the electrode to the mirror surface. The polished electrodes were ultrasonically cleaned 3 times with dilute nitric acid, absolute ethanol, and deionized water, each time for 30 s;

[0029] (2) Put the cleaned electrode in 0.5mol / L dilute sulfuric acid solution for electrochemical activation by cyclic voltammetry, the potential range is -0.5V~+1.2V, the scan rate is 100mV / s, and the cycle The number is 50 laps, and then the electrode is washed with deionized water and dried;

[0030] (3) Place the dried electrode in 1mmol / L K 3 [Fe(CN) 6 ] solution (containing 0.1m...

Embodiment 2

[0037] The preparation method of the polymethylene blue composite material modified electrode described in this example is different from Example 1 in that: in step (4), the pretreated glassy carbon electrode is placed in a solution containing 0.8mM or 1.2mM In the PBS buffer solution with a pH of methylene blue of 7, the electropolymerization reaction was carried out by cyclic voltammetry, the cycle potential was - 0.4 ~ + 1.6 V, the scan rate was 100 mV / s, and the cycle number was 15 cycles to obtain polymethylene blue modified electrode.

Embodiment 3

[0039] The nickel oxide / polymethylene blue composite modified electrode of the present invention is prepared by the following method:

[0040] (1) Weigh 0.86g of sodium lauryl sulfate and put it in a beaker, dissolve it with 80ml of deionized water, add 0.95g of nickel chloride hexahydrate and 0.60g of urea under vigorous stirring, and then transfer the light green mixed solution Put into oven in 100 milliliters of Teflon stainless steel autoclaves, make it react 8h under 160 ℃ of conditions. After naturally cooling to room temperature, pour off the waste liquid, collect the light green precipitate, and centrifuge at 8000 r / min for 10 min. The obtained precipitate was repeatedly washed with deionized water, and then repeatedly washed with ethanol. The washed precipitate was placed in a vacuum drying oven and kept dry at 60 °C for 11 h to prepare nickel oxide nanoflowers. image 3It is the XRD figure of the nickel oxide prepared by the present invention, the product is NiO, a...

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Abstract

The invention relates to the field of electrochemical biosensors, and discloses a preparation method of a modified electrode for glucose detection and the electrode. The preparation method of the modified electrode is as follows: first, the polymethylene blue modified electrode was prepared by cyclic voltammetry, and then the nickel oxide nanoflowers were synthesized by the hydrothermal method, and the dispersion liquid containing nickel oxide was modified on the surface of the polymethylene blue modified electrode by the drop coating method. , A nickel oxide / polymethylene blue composite modified electrode was constructed. The obtained modified electrode is used as the working electrode, the silver / silver chloride electrode is used as the reference electrode, and the platinum electrode is used as the counter electrode to form a three-electrode system. The chronoamperometry is used to detect the glucose solution within a certain concentration range, and the modified electrode It has good selectivity and high sensitivity.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical biosensor, in particular to a preparation method of a composite material modified electrode for detecting glucose and the electrode. Background technique [0002] Diabetes is a chronic disease that seriously endangers people's health and even life. Diabetes can be diagnosed by detecting the concentration of glucose in human blood, reflecting the health status of the human body. Therefore, the in vitro quantitative detection of glucose in blood is of great significance for clinical medical diagnosis and protection of human physical and mental health. [0003] Currently, methods for detecting glucose include spectrophotometry, chromatography, colorimetry, and optical rotation. These methods have problems such as long detection time, cumbersome operation steps, and expensive equipment. Compared with the above-mentioned methods, the electrochemical analysis method has attracted extensive...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327C25D9/02
CPCC25D9/02G01N27/308G01N27/3277
Inventor 王俊朱璐艺韦真博
Owner ZHEJIANG UNIV
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