Lactococcus lactis for expressing mouse antibacterial peptide gene

Abstract

The invention discloses lactococcus lactis for expressing a mouse antibacterial peptide gene, and belongs to the technical field of gene engineering. The nucleotide sequence of a CRAMP protein is optimized, and the constructed lactococcus lactis for expressing the CRAMP protein is used for preparing vaccines for regulating intestinal flora disturbance, so that the lactobacillus lactis has advantages on regulation of intestinal flora and intestinal immune response and maintenance; the whole culture can be directly used as an oral vaccine to stimulate mice and cause strong cellular immune response, and the recombinant lactococcus lactis can be used as a novel oral vaccine product with a good industrial prospect, plays a positive role in relieving intestinal inflammation, and has important practical significance in promoting healthy development of intestinal tracts.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows researchers to create small amounts of proteins called chlorella actinidase (CRAM) from natural sources like Bifdoba cerevisiae cells grown on special media containing specific nutrients such as yeast extract. These tiny particles are then added back into these cultures during fermentations. By controllably inducing gut floral rearrangement by adding certain signals derived from Crameric Aciduria virus type 1 enzymatic activity, we have developed methods for producing effective treatments targeted at preventing diseases associated with excessive gastrointestinal disturbances caused by various factors including stressors.

Problems solved by technology

This patented technical problem addressed in this patent relating to finding ways to prevent diseases caused by harmful organism such as helminthosis through their production of specific types of proteins known collectively as CRAMPs. These molecules were discovered during research into how they could protect tissues like gut walls without causing damage. However, these small polypeptides may still cause inflammatory responses when delivered alone. To address this issue, we developed different strategies based upon introducing them inside the enterocyte lining layer of the host defense mechanism.

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