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51results about How to "Good industry prospects" patented technology

Protein recombinant lactococcus lactis for secretory expression of core antigen COE of PEDV (Porcine Epidemic Diarrhea Virus) as well as preparation method and application of protein recombinant lactococcus lactis

The invention belongs to the field of animal biomedicine engineering, and in particular discloses protein recombinant lactococcus lactis for secretory expression of a core antigen COE of a PEDV (Porcine Epidemic Diarrhea Virus) as well as a preparation method and an application of the protein recombinant lactococcus lactis. The method comprises the following steps: connecting a signal peptide and other sequences onto a gene of the core antigen COE of the PEDV via an overlap extension PCR method by designing multiple primers, connecting the gene to a lactococcus lactis expression vector pNZ8048 and introducing the vector with the gene into a cell of the lactococcus lactis NZ9000 in an electrotransformation manner so as to obtain recombinant bacteria; and inducing the recombinant bacteria with nisin so as to obtain an expression, and directly taking all induced cultures as oral vaccines capable of stimulating mice and inducing strong cellular immune responses. Thus, the protein recombinant lactococcus lactis can serve as a novel oral vaccine product with a good industrial prospect and has a positive effect for reducing harms of the PEDV to pig industry, thereby playing a great practical significance for promoting the healthy development of the pig industry.
Owner:SOUTH CHINA AGRI UNIV

Bispecific chimeric antigen receptor targeting CD123 and NKG2D ligands and application of bispecific chimeric antigen receptor

PendingCN111995688AEnhance specific killing effectOvercoming immunosuppressionVirusesMicroorganism based processesSingle-Chain AntibodiesNkg2d ligands
The invention discloses a bispecific chimeric antigen receptor targeting CD123 and NKG2D ligands and the application of the bispecific chimeric antigen receptor, and particularly discloses a bispecific chimeric antigen receptor (CAR) amino acid construct or a functional variant thereof, wherein the bispecific chimeric antigen receptor (CAR) amino acid construct can simultaneously expresses an anti-CD123 single-chain antibody and a natural NKG2D extracellular fragment and can target CD123 and NKG2DL; the bispecific chimeric antigen receptor has a parallel connection mode and a series connectionmode; and the invention discloses a CAR (chimeric antigen receptor), a construct structure thereof, a nucleotide sequence of the construct, a recombinant expression vector containing the nucleotide sequence and a construction mode and application of the corresponding expression vector. The CAR structure endows T cells with higher and lasting multiplication capacity and high anti-tumor capacity; and according to the bispecific chimeric antigen receptor, multiple infusions of single-target CAR-T cells can be avoided, so that not only is the harm to a patient reduced, but also the economic pressure of the patient can be reduced, and the bispecific chimeric antigen receptor has relatively great clinical research and application values.
Owner:金鑫

Low-carbon quasi-single crystal ingot furnace and method for adopting low-carbon quasi-single crystal ingot furnace for ingot casting

The invention discloses a low-carbon quasi-single crystal ingot furnace and a method for adopting the low-carbon quasi-single crystal ingot furnace for ingot casting. The low-carbon quasi-single crystal ingot furnace comprises a furnace body (1), a heat insulation cage (2) and a heat exchange table (9), wherein a crucible (7) is placed on the heat exchange table (9), the top of the crucible (7) is provided with heaters A (3), and heaters B (8) are distributed at the circumference of the crucible (7) and are connected with traction devices (12) through heater lifting rods (11). The method comprises five steps including material charging, heating melting, crystal growth, annealing and cooling. The low-carbon quasi-single crystal ingot furnace and the method have the advantages that the ingredient supercooling phenomenon caused by carbon content enriching in a solute boundary layer can be effectively inhibited, so the influence on the growth speed of quasi-single crystals caused by impurity enriching is reduced, and meanwhile, the content of carbon in quasi-single crystal ingots can be effectively reduced; and the generation and the growth of the fine crystal regions near the crucible wall can be effectively inhibited, so the quality of the quasi-single crystal ingots can be obviously improved, and the utilization rate of the ingots is improved.
Owner:TIANWEI NEW ENERGY HLDG +1

Combined guard board for preparing pseudo-single crystal with high utilization rate and method for preparing pseudo-single crystal

The invention discloses a combined guard board for preparing a pseudo-single crystal with high utilization rate and a method for preparing the pseudo-single crystal, wherein the guard board is improved to realize different heat radiation intensities in different areas of a crucible, so as to improve a growth condition of the single crystal. The combined guard board for preparing the pseudo-single crystal with high utilization rate comprises a lateral crucible graphite guard board which is attached onto the outer wall of the crucible; the lateral crucible graphite guard board comprises an inner guard board attached onto the outer wall of the crucible, and an outer guard board attached onto one side of the inner guard board; the outer guard board is located on one side of the inner guard board far away from the crucible; a plurality of strip-shaped inner guard board holes with the axes being in parallel with one another are formed on the inner guard board; a plurality of strip-shaped outer guard board holes with the axes being in parallel with one another are formed on the outer guard board; and the axes of the strip-shaped inner guard board holes are in parallel with the axes of the strip-shaped outer guard board holes. With the adoption of the combined guard board, the ratio of the radial transmission to the vertical transmission of the heat from the crucible is greatly decreased so as to effectively inhibit the radial heat current of the crucible, and the formation proportion of polycrystal around a cast ingot is decreased, so that the utilization rate of the cast ingot is increased.
Owner:TIANWEI NEW ENERGY HLDG

Device and method for rainwater collection, treatment and reuse in multi-span greenhouses

The invention discloses a multi-span greenhouse rainwater collecting and treating reusing device which comprises a water gathering pipe, initial rainwater filtering devices, a double power supply programming controller, a safety diverting well, a modular water storage pond, a pressurizing pump, a multi-medium filter, an ozone generator, a Venturi ejector and a clean water pond, wherein the initial rainwater filtering devices are used for initially realizing filtration of rainwater of 2-5mm; the double power supply programming controller is used for controlling the liquid level of the water storage pond and switching of the initial rainwater filtering devices; the safety diverting well is used for precisely filtering rainwater and controlling the water quantity; the ozone generator and the Venturi ejector are used for generating ozone water. The invention further discloses a rainwater collecting and treating reusing method based on the device. According to the device and method disclosed by the invention, the collected rainwater is disinfected and sterilized by using the ozone water so as to solve the problem that the water quality in the water storage pond is deteriorated, so that the rainwater is recycled, and therefore, the utilization efficiency of a water resource is improved, the regional ecological environment is improved, and an extra economical benefit is further generated.
Owner:拜森生物环保科技(上海)有限公司

A kind of iron ore pellet containing biochar and preparation method thereof

The invention relates to biochar-containing iron ore pellets and a preparation method thereof. The preparation method comprises the following steps of: uniformly mixing iron ore powder, biochar, a binder and water to form a mixed material A; uniformly mixing iron ore powder, a binder and water to form a mixed material B; firstly putting the mixed material A in a pelletizer to prepare inner spheres, and then putting the mixed material B to form outer layers for coating the inner spheres, namely obtaining the biochar-containing iron ore pellets containing double-layer sphere structures; and controlling the diameter of the inner spheres to be D1 which is 8-10mm and the diameter of the pellets to be D2, wherein D2-D1 is 1-4mm and D2 is less than or equal to 12mm, the mass ratio of carbon of the biochar in in the inner spheres to oxygen of the iron ore powder is (0.95-1.3): 1, and the mass ratio of carbon of the biochar in the pellets to oxygen of the iron ore powder is (0.63-0.85): 1. Thetesting proves that the compression strength, drop strength and detonative temperature of the biochar-containing iron ore pellets prepared by the method can meet self-reduction pellets production verywell. The biochar-containing iron ore pellets are simple in technology, low in production cost and environmentally-friendly and have good industry foreground.
Owner:CENT SOUTH UNIV
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