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Mahonia bealei (Beale's barberry) is a shrub native to mainland China (Anhui, Fujian, Guangdong, Guangxi, Henan, Hubei, Hunan, Jiangsu, Jiangxi, Shaanxi, Sichuan, Zhejiang). The species has sometimes been regarded as the same species as Mahonia japonica, native to Taiwan, but the two differ consistently in certain floral and leaf characters. Both species are widely cultivated in many countries as ornamentals. Mahonia bealei has reportedly escaped cultivation and become established in the wild in scattered places in the southeastern United States from Arkansas to Florida to Delaware.
Fingerprint detection method of Mahonia bealei and its identification and extraction method
The present application belongs to the technical field of traditional Chinese medicine modernization, and particularly relates to a fingerprint spectrum detection method of Mahonia leaf, and an identification and extraction method thereof. The fingerprint spectrum detection method of Mahonia leaf comprises the following steps: (1) preparation of a reference solution; (2) preparation of a sample solution: taking a Mahonia leaf sample and adding an extraction solvent B for extraction; (3) performing ultra-high performance liquid chromatography analysis on the reference solution and the sample solution; and (4) establishing a fingerprint spectrum of the Mahonia leaf medicinal material. The fingerprint spectrum detection method of Mahonia leaf can identify Mahonia leaf of different origins, and screen common characteristic peaks and / or potential differential components of Mahonia leaf of different origins. In addition, the extraction method can extract index components of Mahonia leaf through column layer analysis. The chemical fingerprint spectrum method of the present application is stable and reliable, can significantly distinguish Mahonia leaf of different origins, and can provide a reference for quality control, evaluation and origin identification of Mahonia leaf, and has important significance for ensuring clinical efficacy.
Owner:TONGJITANG CHINESE MEDICINES CO