Lactobacillus rhamnosus and application in the preparation of antidepressant products

A technology of Lactobacillus rhamnosus and products, applied in the direction of bacteria, Lactobacillus, and applications used in food preparation, can solve problems such as incomplete elucidation, and achieve good industrial prospects

Active Publication Date: 2018-10-30
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the pathogenesis of depression has not been fully elucidated, but through the joint efforts of a large number of scientific researchers, it has been found that the occurrence of depression is caused by changes in complex signaling networks covering the monoamine neurotransmitter system , neuroendocrine system, neurotrophic factors, neurogenesis, changes in the immune system and epigenetic modifications, etc., and genetic factors also contribute considerably to the pathogenesis of depression
[0003] At present, the development of clinical therapeutic drugs is mainly based on the "monoamine hypothesis". This type of drug has no effect on some patients with depression (20-30%). Therefore, it is very important to find more effective and faster methods of prevention and auxiliary treatment of depression. necessary

Method used

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  • Lactobacillus rhamnosus and application in the preparation of antidepressant products
  • Lactobacillus rhamnosus and application in the preparation of antidepressant products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Embodiment 1, isolation, identification and preservation of bacterial strains

[0076] 1. Isolation of strains

[0077] In August 2016, fresh feces of newborn babies were collected from Changchun City, Jilin Province, and the fecal core samples were picked and put into the transport medium, sealed with 1mL sterilized liquid paraffin oil, and quickly placed in an ice box and sent to the laboratory for further investigation separate. After gradient dilution of sauerkraut samples and feces samples, the Lactobacilli in the samples were separated using MRS solid medium and Lactobacillus selective medium respectively, cultured at 37°C for 48 hours under anaerobic conditions, and typical colonies on the plate were picked. Streak culture and isolate pure colonies.

[0078] The isolated strains were subjected to morphological identification, physiological and biochemical identification, and molecular identification. Among them, two strains belonged to Lactobacillus rhamnosus a...

Embodiment 2

[0085] Example 2, the antidepressant function of Lactobacillus rhamnosus TF318

[0086] The tested bacteria were: Lactobacillus rhamnosus TF316 (represented by TF316), Lactobacillus rhamnosus JAAS8 (represented by JAAS8) and Lactobacillus rhamnosus TF318 (represented by TF318).

[0087] 1. Preparation of bacterial suspension

[0088] 1. The test bacteria were inoculated in liquid MRS medium, cultured at 37°C for 16 hours, and then centrifuged at 4°C, 6000rpm for 8min to collect the bacterial precipitate.

[0089] 2. Take the bacterial precipitate obtained in step 1 and suspend it with sterile physiological saline to obtain a bacterial concentration of 2×10 10 CFU / mL bacterial suspension.

[0090] When the test bacteria is TF316, the bacterial suspension obtained by carrying out the above steps is named as TF316 bacterial suspension.

[0091] When the test bacteria is JAAS8, the bacteria suspension obtained by carrying out the above steps is named as JAAS8 bacteria suspensio...

Embodiment 3

[0119] Example 3, verification of antidepressant efficacy of Lactobacillus rhamnosus TF318

[0120] 1. Preparation of bacterial suspension

[0121] 1. Lactobacillus rhamnosus TF318 was inoculated in liquid MRS medium, cultured statically at 37°C for 16 hours, then centrifuged at 4°C and 6000rpm for 8min to collect bacterial precipitates.

[0122] 2. Take the bacterial precipitate obtained in step 1 and suspend it with sterile physiological saline to obtain a bacterial concentration of 2×10 10 CFU / mL of TF318 bacterial suspension.

[0123] Two, group processing

[0124] The experimental animals were clean grade ICR male mice.

[0125] The experimental animals were first subjected to adaptive feeding for 3 consecutive days, and then randomly divided into 4 groups, 30 in each group, and were treated as follows:

[0126] Control group: intragastric administration of normal saline once a day for 21 consecutive days (a single dose of 0.2 mL for a single animal), 18 hours after t...

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Abstract

The invention discloses a lactobacillus rhamnosus and an application in the preparation of antidepressant products. The invention provides a lactobacillus rhamnosus TF318 of which the CCTCC No. is 2018113. The invention also provides the application of lactobacillus rhamnosus TF318 in the preparation of antidepressant products. The invention also provides an antidepressant product of which the active ingredient is lactobacillus rhamnosus TF318. The product is food, health food or drug. The lactobacillus rhamnosus TF318 has antidepressant effect and can be used as a functional probiotic bacteria which is capable of treating and preventing depression. The lactobacillus rhamnosus provides new idea for clinically preventing and controlling spiritual diseases relative to depression.

Description

technical field [0001] The invention relates to a strain of Lactobacillus rhamnosus and its application in preparing antidepressant products. Background technique [0002] Depression is a common, complex, and potentially life-threatening mental illness. So far, the pathogenesis of depression has not been fully elucidated, but through the joint efforts of a large number of scientific researchers, it has been found that the occurrence of depression is caused by changes in complex signaling networks covering the monoamine neurotransmitter system , neuroendocrine system, neurotrophic factors, neurogenesis, changes in the immune system and epigenetic modifications, etc., and genetic factors also have a considerable contribution to the pathogenesis of depression. [0003] At present, the development of clinical therapeutic drugs is mainly based on the "monoamine hypothesis". This type of drug has no effect on some patients with depression (20-30%). Therefore, it is very important...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P25/24A23L33/135C12R1/225
CPCA61P25/24A23L33/135A23V2002/00A61K2035/115C12R2001/225C12N1/205A23V2400/175A23V2200/322
Inventor 李盛钰赵玉娟段翠翠赵子健王超高磊
Owner JILIN ACAD OF AGRI SCI
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