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Mononuclear, binuclear and mature maize pollen separation method and construction and application of pollen development stage judgment model

A technology of corn pollen and pollen, applied in the field of plant biochemistry, can solve the problems of complicated operation, tassel damage, poor accuracy of pollen cell stage judgment, etc., and achieve the effect of simple operation, maintaining activity, and non-toxic density

Active Publication Date: 2020-07-28
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] On the other hand, the present invention also provides a method for constructing a judgment model of corn pollen development stage, which is applied to the rapid determination of the development stage of corn pollen, and realizes accurate numerical quantitative judgment, so as to solve the problem of the judgment accuracy of pollen cell stage in the prior art. poor technical problem
[0008] In yet another aspect, the present invention also explores and establishes a simple and intuitive method for quickly judging the developmental stage of corn pollen, without the need for destructive sampling detection, so as to solve the technical problem of complex operation and the need to destroy tassels in existing methods

Method used

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  • Mononuclear, binuclear and mature maize pollen separation method and construction and application of pollen development stage judgment model
  • Mononuclear, binuclear and mature maize pollen separation method and construction and application of pollen development stage judgment model
  • Mononuclear, binuclear and mature maize pollen separation method and construction and application of pollen development stage judgment model

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Embodiment 1

[0045] The separation and purification method of maize mononuclear, dinuclear and mature pollen, see figure 1 , including the following steps:

[0046] (1) According to the number of leaf rings and the length of spikelets, the development stage of the pollen can be roughly judged, and the corn tassels are taken (for early tassels that are still wrapped in the top leaves, the corn tassels wrapped in leaves can be carefully dissected. ), put them in a ziplock bag and store them on ice, and according to the length of the spikelets on the tassels, take the spikelets near the middle of the tassels, put them into a juice extractor, add 4°C pre-cooled grinding buffer, and grind for 12 ~15 s (slightly oscillate up and down for grinding, pause once every 3s, and grind 4 times);

[0047] (2) Use different mesh sieves (if you want to separate and purify mononuclear pollen, choose 300 mesh sieve, use 230 mesh sieve when separating dinuclear pollen, use 150 mesh sieve when separating matu...

Embodiment 2

[0055] Experimental Study on the Correlation Between Leaf Ring Number, Spikelet Length on Tassel, Pollen Diameter and Pollen Development Stage in Maize Plants

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Abstract

The invention discloses a mononuclear, binuclear and mature maize pollen separation method and construction and an application of a pollen development stage judgment model. A compatible sample grinding-separation-purification buffer system is reasonably arranged in the pollen separation and purification method, so that the activity of pollen cells is effectively kept, and implementation of subsequent steps is facilitated; according to the method, the relevance among the corn plant leaf ring number, the tassel middle spikelet length, the pollen diameter and the pollen development stage is foundthrough research, a quantitative numeralization judgment model of the corn pollen development stage and a simple, convenient and rapid intuitive judgment method are established separately, wherein the model judgment method can accurately judge the development stage of the corn pollen; and the intuitive judgment method does not damage the corn tassels and the wrapped leaves (indexes such as leaf ring number and spikelet length are easy to inspect and obtain), is simple and visual to operate, and solves the technical problem that the corn tassels are damaged for sure (if the tassels wrapped bythe leaves need to be taken out, the leaves are damaged for sure) in the traditional judgment method.

Description

[0001] The invention relates to the technical field of plant biochemistry, in particular to a single-core, two-core and mature corn pollen separation method and the construction and application of a pollen development stage judgment model. Background technique [0002] The development process of maize pollen can be divided into two stages: microsporogenesis and male gametophyte. Microsporogenesis begins with mitosis of stamen primordia and ends with meiosis of microspore mother cells to produce microspores. Primary sporogenous cells undergo mitosis to generate pollen mother cells, which initiate meiosis and produce four haploid microspores, which are released as the callose wall disintegrates. Male gametogenesis begins with the release of microspores and ends with the formation of sperm cells; the polarized microspores undergo a special asymmetric mitosis that produces a large vegetative cell and a small germ cell (dinucleate pollen). The germ cell enters the second mitosis, p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04G01N33/48G06Q10/04G06Q50/02G06Q10/06
CPCC12N5/04G01N33/4833G06Q10/04G06Q10/067G06Q50/02
Inventor 杨浩苏磊赵霞胡秀丽穆心愿曹言勇李雪王志飞
Owner HENAN AGRICULTURAL UNIVERSITY
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