Application of polypeptide Kp-10 in preparation of medicine for treating and preventing osteosarcoma

A technology of kp-10, osteosarcoma, applied in the field of biomedicine

Pending Publication Date: 2020-07-31
SHANGHAI CHANGZHENG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether KiSS-1 / GPR54 plays a role in inhibiting osteosarcoma has not yet been reported, and its role in the preparation of drugs that inhibit the development of osteosarcoma and lung metastasis is also in urgent need of development and research

Method used

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  • Application of polypeptide Kp-10 in preparation of medicine for treating and preventing osteosarcoma
  • Application of polypeptide Kp-10 in preparation of medicine for treating and preventing osteosarcoma
  • Application of polypeptide Kp-10 in preparation of medicine for treating and preventing osteosarcoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This example verifies that the expression of GPR54 / KiSS-1 in osteosarcoma tissues is significantly lower than that in adjacent normal tissues. The specific experimental contents and results are as follows:

[0028] Using the human osteoblast cell line HFOB1.19 as a normal control, the collected human osteosarcoma tissue specimens were stained and analyzed by immunohistochemical staining, and the results were as follows: figure 1 shown. Among them, 143B and MNNG / HOS cell lines are both derived from osteosarcoma TE85 cell line, and 143B cells have stronger metastatic activity after introducing ki-ras gene; ZOS and ZOSM cell lines are derived from in situ and distant metastatic lesions of the same patient. Depend on figure 1 It can be seen that the expression of GPR54 / KiSS-1 in osteosarcoma patient tissues was significantly lower than that in adjacent normal tissues; taking the human osteoblast cell line HFOB1.19 as a normal control, the expression of KiSS-1 and GPR54 in ...

Embodiment 2

[0030] This example verifies that the expression of GPR54 / KiSS-1 is highly correlated with the clinical prognosis of patients with osteosarcoma. The specific experimental content and results are as follows:

[0031] By counting the expression level of GPR54 / KiSS-1, combined with the clinical follow-up data of patients, the following figure 2 the result of. Depend on figure 2 It can be seen that the expression of GPR54 / KiSS-1 is highly correlated with the clinical prognosis of patients with osteosarcoma, that is, the lower expression of GPR54 / KiSS-1 indicates that the survival period of patients is shorter.

Embodiment 3

[0033] This embodiment verifies the inhibitory effect of GPR54 / KiSS-1 on the proliferation and migration of osteosarcoma cell lines MNNG / HOS and ZOS cells. The specific experimental content and results are as follows:

[0034] The effect of Kp-10 (the shortest active polypeptide in KiSS protein) on the proliferation and migration of osteosarcoma cell lines MNNG / HOS and ZOS cells was studied by colony formation and transwell experiments. The results are as follows: image 3 shown. Knock down the GPR54 gene to study the changes in the proliferation and migration abilities of MNNG / HOS and ZOS cells, the results are as follows Figure 4 shown.

[0035] Depend on image 3 It can be seen that Kp-10 can significantly inhibit the proliferation and migration of osteosarcoma cell lines MNNG / HOS and ZOS cells in a dose-dependent manner. Depend on Figure 4 A shows that the expression level of GPR54 in the knockdown group decreased; by Figure 4B shows that compared with the blank co...

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Abstract

The invention discloses an application of a polypeptide Kp-10 in preparation of a medicine for treating and preventing osteosarcoma. The amino acid sequence of the polypeptide Kp-10 is DSSDSSDSSDSSDSSDSSYNWNSFGLRF. Researches find that expression of GPR54/KiSS-1 in osteosarcoma tissues is obviously lower than that in normal tissues beside the tumor tissues, and is highly related to clinical prognosis, and the polypeptide Kp-10 and a receptor GPR54 of the polypeptide Kp-10 can inhibit proliferation and migration capacity and lung metastasis capacity of osteosarcoma cells, so that the polypeptide Kp-10 can be used for preparing medicines for treating and preventing osteosarcoma, and a new thought is provided for exploration of osteosarcoma targeted medicine.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of a polypeptide Kp-10 in the preparation of medicines for treating and preventing osteosarcoma. Background technique [0002] Osteosarcoma is the most common type of bone malignancy and is a malignant osteogenic tumor originating from mesenchymal cells. Most of the patients are teenagers, and most of them are men. It is more likely to occur in the skull. The frontal bone is the most common in the skull, followed by the parietal bone, temporal bone, and occipital bone. In the facial bones, it is mostly located in the maxilla, mandible, zygomatic bone, and nasal bone, followed by the frontal sinus, orbital bone, etc., and the tibial bone. 1 / 3 of the front side. Tumors grow slowly and have mild symptoms. They mostly appear in childhood and gradually grow with physical development. When they are 10 to 20 years old, after several years or decades of disease course, most o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P19/08A61P35/00A61P35/04
CPCA61K38/17A61P19/08A61P35/00A61P35/04
Inventor 肖建如李珍惜王冬生何韶辉茹炳华孙海涛魏海峰吴志鹏赵剑钱明
Owner SHANGHAI CHANGZHENG HOSPITAL
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