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Sputum pathogenic microorganism metagenome de-homing extraction system and method

A technology of pathogenic microorganisms and metagenomics, which is applied in the field of sputum pathogenic microorganisms metagenomics dehostization extraction system, can solve the problems of long process, high sample requirements and high cost, and achieve mild reaction, good cracking effect and short reaction time Effect

Pending Publication Date: 2020-07-31
上海珈湘生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The former has a lower cost, but has higher requirements for samples, and cannot process sputum samples temporarily; the latter has no selectivity for samples, but has higher cost, longer process, and poorer effect

Method used

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  • Sputum pathogenic microorganism metagenome de-homing extraction system and method
  • Sputum pathogenic microorganism metagenome de-homing extraction system and method
  • Sputum pathogenic microorganism metagenome de-homing extraction system and method

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Embodiment Construction

[0042] In order to make the content of the present invention clearer and easier to understand, the content of the present invention will be further described below in conjunction with specific embodiments of the specification. Of course, the present invention is not limited to this specific embodiment, and general replacements known to those skilled in the art are also covered within the protection scope of the present invention.

[0043] The present invention will be described in further detail below in conjunction with specific examples.

[0044] The sputum pathogenic microorganism metagenome dehostization extraction system in this embodiment includes: a sputum liquefaction module; a dehostization module; and a nucleic acid extraction module. The sputum liquefaction module is used to liquefy the sputum; the host removal module is used to remove the host; the nucleic acid extraction module is used to extract the nucleic acid after removing the host.

[0045] Here, the sputum...

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Abstract

The invention provides a sputum pathogenic microorganism metagenome de-homing extraction system and method. The system comprises a sputum liquefaction module for liquefying sputum, a de-homing modulefor removing a host, and a nucleic acid extraction module used for extracting nucleic acid from which the host is removed. According to the invention, a sputum sample can be treated in advance in combination with a host removal system, and nucleic acid extraction is carried out in combination with an extraction kit. The complete characteristics of pathogenic microorganisms in sputum are reserved as much as possible while sputum liquefaction is met, so that the sputum liquefaction and removal of human-derived hosts are realized, and genomes of microorganisms are highly reserved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a system and method for extracting host-free metagenomes of pathogenic microorganisms from sputum. Background technique [0002] As a common respiratory sample, sputum samples are widely used in the detection of pathogenic microorganisms in respiratory tract infections. However, because sputum contains a large amount of mucus, cells and proteins, it is difficult to release a large number of pathogenic microorganisms wrapped in sputum. At the same time, Sputum contains a large number of human-derived cells, which causes a large amount of background interference in the targeted detection of pathogenic microbial nucleic acids, which affects both mNGS and third-generation sequencing technologies. A large amount of data is used for human-derived detection instead of pathogenic microorganisms. Therefore, the removal of human hosts can greatly improve the detection sensitivity in mNGS and t...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 丁力
Owner 上海珈湘生物科技有限公司