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A kind of extraction method of limonin

A technology of limonin and an extraction method, which is applied in the directions of food ingredients containing natural extracts, functions of food ingredients, steroids, etc., can solve the problems that can not be directly used as medicine, lack of asphalt-based waterproof rubber compound, etc.

Active Publication Date: 2021-04-27
广州市莱檬生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method adds chemical solvents such as acetone and chloroform as extracts, and the obtained product cannot be directly used as medicine, especially as food and medicine, which is directly taken internally by the human body, and needs further improvement
[0005] In summary, the prior art still lacks a bitumen-based waterproof adhesive with high extraction efficiency and good extraction method

Method used

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  • A kind of extraction method of limonin
  • A kind of extraction method of limonin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for extracting limonin, comprising the following steps:

[0031] (1) Pretreatment, soak 10kg of lemon seeds and lemon peel residue in 200kg of water, add 10g of Celluclast 1.5L cellulase, 5g of subtilisin, and 5g of 1,4-α-D-glucanohydrolase to decompose impurities;

[0032] (2) Extraction, add 2g of Novozymes Celluclast 1.5L cellulase to the pretreated product, treat for 1 hour, then add nitric acid for 1 hour, and the pH of the reaction system during acidolysis is 3;

[0033] (3) solid-liquid separation, using a plate and frame filter to remove solid residues, leaving liquid to obtain the initial product;

[0034] (4) Remove pectin, concentrate the initial product in vacuum, measure the sugar content of the concentrated solution by a hand-held refractometer sugar meter, stop the vacuum concentration when the sugar content reaches 8%, and then use 98% ethanol, 94% isopropanol mixed solution to carry out alcohol precipitation, The volume ratio of ethanol to iso...

Embodiment 2

[0037] The difference between this embodiment and embodiment 1 is that the extraction process is not used.

[0038]A method for extracting limonin, comprising the following steps:

[0039] (1) Pretreatment, soak 10kg of lemon seeds and lemon peel residue in 200kg of water, add 10g of Celluclast 1.5L cellulase, 5g of subtilisin, and 5g of 1,4-α-D-glucanohydrolase to decompose impurities;

[0040] (2) Extraction, add 1g of Novozymes Celluclast 1.5L cellulase to the pretreated product, treat for 2 hours, then add nitric acid for 2 hours, and the pH of the reaction system during acidolysis is 4;

[0041] (3) solid-liquid separation, using a plate and frame filter to remove solid residues, leaving liquid to obtain the initial product;

[0042] (4) Remove pectin, concentrate the initial product in vacuum, measure the sugar content of the concentrated solution by a hand-held refractometer sugar meter, stop the vacuum concentration when the sugar content reaches 8%, and then use 98% ...

Embodiment 3

[0045] The main difference between this embodiment and Example 1 is that the initial product will be left to stand for 7 days in step (3).

[0046] A method for extracting limonin, comprising the following steps:

[0047] (1) Pretreatment, soak 10kg of lemon seeds and lemon peel residue in 200kg of water, add 10g of Celluclast 1.5L cellulase, 5g of subtilisin, and 5g of 1,4-α-D-glucanohydrolase to decompose impurities;

[0048] (2) Extraction, add 2g of Novozymes Celluclast 1.5L cellulase to the pretreated product, treat for 1 hour, then add nitric acid for 1 hour, and the pH of the reaction system during acidolysis is 3;

[0049] (3) Solid-liquid separation, using a plate and frame filter to remove solid residues, leaving liquid to obtain the primary product, which will be left to stand for 7 days;

[0050] (4) Remove pectin, concentrate the initial product in vacuum, measure the sugar content of the concentrated solution by a hand-held refractometer sugar meter, stop the va...

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Abstract

The invention discloses a method for extracting limonin, which comprises the following steps: pretreatment, adding lemon seeds and lemon peel residues to soak in water, adding pretreatment enzymes to decompose impurities; extraction, adding pretreatment products to extracting enzymes for extraction Enzymatic hydrolysis, add acid at the same time for acid hydrolysis, solid-liquid separation, remove solid residue, leave liquid, and obtain the primary product; remove pectin, vacuum concentrate the primary product, add alcohol to precipitate, separate the floc precipitation, and leave the liquid ; Purification, first extraction, and then use macroporous resin separation. The extraction method of the present invention is reasonably designed, each step is interrelated, removes impurities one by one, extraction and macroporous resin separation are used in combination, and extraction is performed first and then macroporous resin separation, combining the advantages of the two separations, the separation efficiency is high, and the product High purity.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, relates to the technical field of extraction of plant natural medicinal components, in particular to a limonin extraction method and application thereof. Background technique [0002] Limonin, also known as Phellodendron lactone, Evodicarlide, has various biological activities such as anti-tumor, insect antifeedant, anti-virus, analgesic, anti-inflammatory, hypnotic, etc. It is an important natural pharmaceutical raw material and can be used for the treatment of Pneumonia, functional food additives, anti-cancer food, pesticides, feed additives, etc. At present, the extraction and separation of limonin adopts the methods of multiple extractions, multiple column chromatography and preparation of liquid phases. This extraction method is relatively cumbersome and the extraction rate is low. [0003] CN102442916A discloses a method for separation and purification of limonoid compound C in Tianguo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J73/00A61P1/16A61P11/00A23L33/105
CPCA23V2002/00A23L33/105A61P1/16A61P11/00C07J73/008A23V2200/30A23V2250/21
Inventor 廖劲松
Owner 广州市莱檬生物科技有限公司