Heterotrophic nitrification-aerobic denitrification pseudomonas and culture method and application thereof

An aerobic denitrification and pseudomonas technology, applied in the field of microorganisms, can solve the problems of narrow nitrogen concentration range, low nitrogen load, poor denitrification performance, etc., and achieve the effect of high nitrogen rate

Active Publication Date: 2020-08-14
GUANGDONG UNIV OF PETROCHEMICAL TECH
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] Although there are many studies on HN-AD bacteria, which play an increasingly important role in the field of sewage treatment, there are still some problems, such as the na

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  • Heterotrophic nitrification-aerobic denitrification pseudomonas and culture method and application thereof
  • Heterotrophic nitrification-aerobic denitrification pseudomonas and culture method and application thereof
  • Heterotrophic nitrification-aerobic denitrification pseudomonas and culture method and application thereof

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Embodiment Construction

[0040] The present invention will be further described below in conjunction with specific preferred embodiment, but does not limit protection scope of the present invention thereby.

[0041] Source of the strain: The source of the strain was the activated sludge collected from the Longjin Sewage Plant in Longyan City, Fujian Province. Through enrichment, isolation and purification, primary screening and secondary screening, YG5 with HN-AD performance was selected as the target strain. YG5 adopts inclined-plane preservation and cryopreservation methods to preserve strains. For short-term use, the strains should be stored in beef extract peptone slant at 4°C, and for long-term use, they should be stored in glycerin suspension at -80°C.

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Abstract

The invention discloses a heterotrophic nitrification-aerobic denitrification pseudomonas. The strain is Pseudomonas sihuiensis YG5, and is preserved in the Guangdong Microbial Culture Collection Center, the preservation time is December 25, 2018, and the preservation number is GDMCC No: 60533. The strain can tolerate high concentrations of NH4<+>-N, NO3<->-N and NO3<->-N and has a high removal rate on NH4<+>-N, NO3<->-N and NO3<->-N in a high concentration range, which indicates that the strain YG5 has the potential of treating wastewater with high concentrations of NH4<+>-N, NO3<->-N and NO3<->-N.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a heterotrophic nitrifying-aerobic denitrifying Pseudomonas and its cultivation method and application. Background technique [0002] With the rapid development of the economy, people's over-exploitation of the natural environment and the rapid development of industry and agriculture have caused serious pollution of natural water bodies, among which nitrogen is the main pollutant of water bodies, which mainly exist in four forms, namely organic nitrogen , ammonia nitrogen, nitrite nitrogen and nitrate nitrogen. Excessive concentration of ammonia nitrogen in the water body will lead to eutrophication of the water body; excessive concentration of nitrate nitrogen will cause human hypertension, methemoglobin and other diseases; nitrate can be converted into nitrite in the body, which can easily induce various diseases and even cause cancer. Therefore, solving the problem of ...

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/38
CPCC12N1/20C02F2101/38C12R2001/38C12N1/205Y02W10/10
Inventor 夏春雨孙巍苏全榕阙才英韦明肯林彩琴魏登枭
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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