A strain of Paenibacillus polymyxa and its application
A technology of Paenibacillus polymyxa and bacillus, applied to Paenibacillus polymyxa strains and its application fields, can solve problems such as unscientific use, drug resistance, ecological environment pollution, food safety, etc., achieve high antibacterial activity, delay pathogenic bacteria resistance Medicinal properties and the effect of reducing dosage
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Embodiment 1
[0042] Isolation and identification of Paenibacillus polymyxa LQ19
[0043] 1. Isolation of Paenibacillus polymyxa strain LQ19
[0044] Collect rhizosphere soil samples from a greenhouse where cucumbers are grown in Wuqing District, Tianjin, put the soil samples into a small triangular flask containing sterile water and sterilized glass beads, and shake at 200 rpm / min for 20 minutes; Serial dilution, 10 3 , 10 4 Pipette 200 μL of the doubling-diluted bacterial suspensions to evenly coat the NA medium, and after culturing at 28°C for 12 hours, pick a single colony, and purify the bacteria by streaking on the NA medium plate. One of the isolated and purified strains was named LQ19.
[0045] 2. Identification of Paenibacillus polymyxa strain LQ19
[0046] 1. Observation of morphological characteristics
[0047] Refer to "Berger's Manual of Systematic Bacteriology" (Garrity, 2001) and "Common Identification Methods of General Bacteriology" (Dong Xiuzhu et al., 2001), and foll...
Embodiment 2
[0053] Bacteriostatic effect of Paenibacillus polymyxa strain LQ19 on 13 pathogenic bacteria
[0054] A. Test pathogens
[0055] The tested pathogenic oomycetes include tomato damping off bacteria; the tested pathogenic fungi include coriander root rot fungus, celery stem base rot fungus, strawberry fusarium wilt, cucumber fusarium wilt, corn stem base rot fungus, cucumber target spot fungus, pepper anthracnose fungus, Cucumber Botrytis cinerea, Sclerotinia sclerotiorum, tomato Botrytis cinerea and celery leaf spot; the tested pathogenic bacteria included Cucumber bacterial angular spot. The above test strains are all isolated and preserved by the Seedling Disease Research Laboratory of Tianjin Plant Protection Research Institute, and can be freely provided for use in the relevant experiments of the present invention, and cannot be used for other purposes.
[0056] B. Test method:
[0057] Method 1: In this experiment, the mycelial growth rate method was used to determine th...
Embodiment 3
[0063] Influence of the strain LQ19 of the present invention on the growth of cucumber
[0064] The strain LQ19 suspension agent of the present invention is diluted to 10 with clear water 7 cfu / mL, soaked the cucumber seeds for 24 hours, and then sowed the soaked seeds in plastic flower pots (8cm in diameter) with nutrient soil, and placed them in a 25°C light incubator for 12 hours. The control group was soaked with water. treatment with 3 replicates for each treatment. Record the emergence rate, plant height, root length and fresh weight after 14 days of treatment, see Table 2 and Table 2 for details. image 3 .
[0065] 7d after sowing, 5 pots of cucumber seedlings were taken out from the control group and used at a concentration of 10 7 cfu / mL strain LQ19 was used to treat cucumber seedlings by root irrigation, and the amount of bacterial solution per pot was 15 mL. After 7 days of treatment, the emergence rate, plant height, root length, and fresh weight were recorded....
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