Paenibacillus polymyxa strain and application thereof

A technology of Paenibacillus polymyxa and bacillus, applied to Paenibacillus polymyxa strains and its application fields, can solve problems such as unscientific use, drug resistance, ecological environment pollution, food safety, etc., achieve high antibacterial activity, delay pathogenic bacteria resistance Medicinal properties and the effect of reducing dosage

Active Publication Date: 2020-08-18
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of root and stem rot of crops is mainly controlled by chemical agent seed treatment or root irrigation, and the main method of leaf disease is controlled by chemical agent spraying. Although chemical agents have outstanding advantages in disease control, their unscientific use has caused resistance Problems such as drug properties, ecological environment pollution and food safety are becoming more and more prominent

Method used

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  • Paenibacillus polymyxa strain and application thereof
  • Paenibacillus polymyxa strain and application thereof
  • Paenibacillus polymyxa strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Isolation and identification of Paenibacillus polymyxa LQ19

[0043] 1. Isolation of Paenibacillus polymyxa strain LQ19

[0044] Collect rhizosphere soil samples from cucumber greenhouses in Wuqing District, Tianjin, put the soil samples into a small triangular flask filled with sterile water and sterilized glass beads, shake at 200rpm / min for 20min; take the shaken suspension to make Serial serial dilution, 10 3 、10 4 Draw 200 μL of the double-diluted bacterial suspension and evenly spread the NA medium. After culturing at 28°C for 12 hours, pick a single colony and purify the strains on the NA medium plate by streaking. One of the isolated and purified strains was named LQ19.

[0045] 2. Identification of Paenibacillus polymyxa strain LQ19

[0046] 1. Observation of Morphological Characteristics

[0047] Refer to "Bergey's Handbook of Systematic Bacteriology" (Garrity, 2001) and "Common Identification Methods in General Bacteriology" (Dong Xiuzhu et al., 2001), a...

Embodiment 2

[0053] Bacteriostatic effect of Paenibacillus polymyxa strain LQ19 on 13 kinds of pathogenic bacteria

[0054] A. The pathogenic bacteria tested

[0055] The pathogenic oomycetes for testing include tomato damping-off fungi; the pathogenic fungi for testing include coriander root rot fungus, celery stem rot fungus, strawberry wilt fungus, cucumber wilt fungus, corn stem rot fungus, cucumber target spot fungus, pepper anthracnose fungus, Cucumber Botrytis cinerea, Sclerotinia sclerotiorum, tomato gray leaf spot and celery leaf spot; the pathogenic bacteria tested included bacterial angular spot of cucumber. The above tested strains are all isolated and preserved by the Seedling Disease Research Office of Tianjin Institute of Plant Protection, and can be provided free of charge for use in related experiments of the present invention, and cannot be used for other purposes.

[0056] B. Test method:

[0057] Method 1: In this experiment, the mycelium growth rate method was used t...

Embodiment 3

[0063] The influence of bacterial strain LQ19 of the present invention on the growth of cucumber

[0064] Bacterial strain LQ19 suspension concentrate of the present invention is diluted to 10 with clear water 7 cfu / mL, soak the cucumber seeds for 24 hours, then sow the soaked seeds in plastic flowerpots (diameter 8cm) with nutrient soil, and culture them in a light incubator at 25°C for 12 hours, and soak the seeds in water for the control group treatment, each treatment set up 3 replicates. Record emergence rate, plant height, root length, fresh weight after processing 14d, see table 2 and image 3 .

[0065] 7 days after sowing, take out 5 pots of cucumber seedlings from the control group, and use a concentration of 10 7 Cucumber seedlings were treated with cfu / mL bacterial strain LQ19 root-irrigating, and the amount of bacterial solution per pot was 15 mL. After 7 days of treatment, the emergence rate, plant height, root length, and fresh weight were recorded. See Table...

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Abstract

The invention discloses a paenibacillus polymyxa strain and application thereof. The strain is paenibacillus polymyxa LQ19, and the preservation number is CGMCC NO. 19097. The bacterial strain disclosed by the invention is wide in antibacterial spectrum, the antibacterial effect on root rot pathogens of horticultural crops and field crops is as high as 73% or above, the antibacterial effect on fungal and bacterial pathogens of crop leaves is as high as 78% or above, and the bacterial strain can be used for preventing and treating soil-borne diseases and air-borne diseases. Meanwhile, the strain disclosed by the invention is safe to crops, can promote seed germination and crop growth, has good drug effect persistence, is not easy to cause drug resistance, can effectively reduce the use amount of chemical pesticides, is beneficial to pollution-free production of crops, and has a very wide application prospect in biological prevention and control of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of fungicides, and relates to a strain of Paenibacillus polymyxa and its application. Background technique [0002] Guaranteeing national food security and ecological security is an overall major strategic issue related to my country's national economic development and social stability. Scientifically carrying out plant protection work is the key to increasing agricultural production and generating income. Crop root rot disease is difficult to prevent and control. It is mostly caused by Fusarium and Pythium. It manifests as rhizome discoloration and rot. In severe cases, crops will stumble or wither and die, threatening the healthy growth of crops. At present, the control of root and stem rot of crops is mainly controlled by chemical agent seed treatment or root irrigation, and the main method of leaf disease is controlled by chemical agent spraying. Although chemical agents have outstanding advantages in d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/60C05G3/00C05G3/50C05G3/70A01N63/25A01P3/00A01P1/00A01P21/00C12R1/01
CPCC12N1/20C05G3/60C05G3/00C05G3/50C05G3/70A01N63/25C12R2001/01C12N1/205
Inventor 杨利娟高苇张春祥王勇
Owner 天津市农业科学院
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