A kind of enzyme-labeled secondary antibody complex and preparation method thereof

An enzyme-labeled secondary antibody and complex technology, applied in the field of immunoassay, can solve the problems of limited number of enzymes and antibodies, insufficient sensitivity, low penetration, etc., to reduce hydrodynamic volume, improve sensitivity, and improve penetration Penetration effect
CN111551709BActive Publication Date: 2021-04-02河南赛诺特生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
河南赛诺特生物技术有限公司
Publication Date
2021-04-02

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Abstract

The invention relates to an enzyme-labeled secondary antibody compound and a preparation method thereof, and belongs to the technical field of immunoassay. The enzyme-labeled secondary antibody compound comprises a polymer skeleton and a labeling enzyme. The labeling enzyme is connected to the polymer skeleton through a coupling agent, and a secondary antibody is connected to the labeling enzyme through a coupling agent; wherein the polymer skeleton is provided with a branched chain end part and a middle branch structure connected with the branched chain end part, and the labeling enzyme is connected to the branched chain end part and the middle branch structure of the polymer skeleton through a coupling agent; and the polymer skeleton is a hyper-branched polymer skeleton. The branched chain end part and the middle branch structure of the polymer skeleton can be connected with a coupling agent, so that the sensitivity of the enzyme-labeled secondary antibody compound is favorably improved, and the accumulation mode of enzyme and antibody is favorable for improving the polymerization degree of enzyme and antibody in the polymer, so that the sensitivity of the enzyme-labeled secondary antibody compound in immunoassay is greatly improved.
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Description

Technical field

[0001] The present invention relates to an enzyme-laid secondary anti-complex and a preparation method thereof, belonging to the technical field of immunoassay.Background technique

[0002] Immunoassay, that is, the method of detecting samples of protein biomarkers by "antibody-antigen specific binding". Immunoassays can be widely used in the qualitative and quantitative analysis of various biological samples due to their height sensitivity and specificity. Methods of immunoassay include: Enzyme-linked immunization (ELISA), Western-blot, immunohistochemistry, lateral flow analysis method, protein array, cellular immunity ( Immunocytochemistry, etc. Wherein "HRP secondary antibody complex" is widely used to detect biomarkers in the field of immunoassays, including its applications immunohistochemistry, enzyme immunoassay, immunoblotting, and other immune cells, "HRP secondary antibody complex" the principle of the sample is identified by a specific antibody complex in the t...

Claims

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