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A test strip for risk detection of malignant progression of esophageal precancerous lesions or early screening of esophageal cancer

A technology for risk detection and esophageal cancer, applied in the field of medicine and biology

Active Publication Date: 2020-12-01
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For more than ten years, more and more research results and application examples of quantum dots have emerged in the field of biomarkers, but there are no related invention reports in the field of diagnostic test strips in the field of malignant progression of precancerous lesions of esophageal cancer and early screening of esophageal cancer

Method used

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  • A test strip for risk detection of malignant progression of esophageal precancerous lesions or early screening of esophageal cancer
  • A test strip for risk detection of malignant progression of esophageal precancerous lesions or early screening of esophageal cancer
  • A test strip for risk detection of malignant progression of esophageal precancerous lesions or early screening of esophageal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Embodiment 1: the preparation of reagent strip

[0080] 1. Experimental materials

[0081] PSMC4 monoclonal antibody (purchased from Santa Cruz Biotechnology Company, product number: sc-166003), ALDOC monoclonal antibody (purchased from Abcam Company, product number: ab87122) and TXLNA monoclonal antibody (Santa Cruz Biotechnology company, product number: sc-80994); PSMC4 polyclonal antibody (Leading Biology Company, catalog number APR07165G), ALDOC polyclonal antibody (Leading Biology Company, catalog number APG01815G), TXLNA polyclonal antibody (LSBio Company, catalog number LS-C314712) and rabbit anti-mouse IgG (Abcam Company, catalog number ab6728).

[0082] PVC bottom plate, nitrocellulose membrane, glass fiber membrane, absorbent paper etc. are all existing commercially available products.

[0083] 2. Preparation of binding pads

[0084] (1) Activation of quantum dots: N-hydroxysulfosuccinimide is used to activate the carboxyl groups on the surface of CdSe / ZnS ...

Embodiment 2

[0102] Example 2: Value analysis of test strips for early screening of esophageal cancer

[0103] The test strips prepared in Example 1 of the present invention were used to detect the serum of patients with esophageal cancer and precancerous lesions to verify the expression levels of PSMC4 gene, ALDOC gene and TXLNA gene in the serum of patients with esophageal cancer and precancerous lesions.

[0104] 1. Sample source

[0105] Serum samples from 400 cases were collected from the Key Open Laboratory of Esophageal Squamous Cell Carcinoma in Henan Province, the First Affiliated Hospital of Zhengzhou University. The 400 serum samples included 200 serum samples from patients with early esophageal cancer (esophageal cancer group) and 200 serum samples from patients with precancerous lesions of the esophagus (precancerous lesion group). The sera of 200 patients with esophageal cancer (esophageal cancer group) were all from newly diagnosed patients who were diagnosed by endoscopy a...

Embodiment 3

[0116] Example 3: Analysis of the value of test strips for risk detection of malignant transformation of esophageal precancerous lesions

[0117] In order to further study the value of the test strip in the risk detection of malignant transformation of precancerous lesions of esophageal cancer, endoscopic diagnostic tests were selected in high-incidence areas of esophageal cancer (Linzhou City, Anyang City, Huixian City, Henan Province, and Changzhi City, Shanxi Province). For 10,000 patients with precancerous lesions of the esophagus, aged 40-70 years, the peripheral blood of 10,000 patients with precancerous lesions was collected, centrifuged to get serum, and then the test strip prepared in Example 1 of the present invention was used to test 10,000 patients with precancerous lesions The serum is tested, and the high-risk group of canceration (the test strip test result is positive, that is, the high-risk group of canceration) and the non-high-risk group of canceration (the t...

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Abstract

The invention belongs to the field of medical biotechnology, and discloses a test strip for detecting the risk of malignant progression of precancerous lesions of the esophagus or for early screening of esophageal cancer. Antibody, the capture antibody is a mixture of quantum dot-labeled PSMC4 antibody, quantum dot-labeled ALDOC antibody and quantum dot-labeled TXLNA antibody; three detection lines, one quality control line and three detection lines are arranged in sequence along the flow direction of the sample on the chromatography pad The coated detection antibodies are PSMC4 polyclonal antibody, ALDOC polyclonal antibody, and TXLNA polyclonal antibody; the quality control line is coated with rabbit anti-mouse IgG. The test strip of the present invention can quickly detect PSMC4, ALDOC, and TXLNA antigens in human serum, realize the combined detection of three tumor markers, and predict the risk of disease progression in patients with esophageal precancerous lesions after 5 years according to the detection results. The operation is simple and easy to use. convenient.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a test strip used for risk detection of malignant progression of precancerous lesions of the esophagus or early screening of esophageal cancer. Background technique [0002] Esophageal cancer (also known as esophageal squamous cell carcinoma) is one of the ten most common malignant tumors in the world. my country is a country with a high incidence of esophageal cancer in the world, and the tissue type is mainly squamous cell carcinoma. According to the latest data from the "China Tumor Registration Annual Report" published in 2018, esophageal cancer ranks sixth in the incidence of malignant tumors and fourth in death in my country, especially Lin County in Henan, Ci County in Hebei and Changzhi in Shanxi are high incidence areas of esophageal cancer. Compared with other regions, the incidence of esophageal cancer is 500:1. It poses a serious threat to peo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/558
CPCG01N33/57407G01N33/57488G01N33/558G01N33/573G01N33/588G01N33/577G01N2333/988
Inventor 王立东杨苗苗赵学科宋昕毛伟敏吉佳佳李恩民雷玲玲韩雪娜范宗民王苒李贝王盼盼裘宋良
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV