Esophageal tissue and/or organoid compositions and methods of making same
A technology of esophagus and organs, applied in biochemical equipment and methods, drug combination, tissue culture, etc., can solve difficult mouse modeling problems
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[0086] The following non-limiting examples are provided to further illustrate embodiments of the invention disclosed herein. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent approaches that have been found to function well in the practice of the invention, and thus can be considered to constitute examples of modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.
[0087] Wnt and retinoic acid signaling control anterior versus posterior foregut fate
[0088] To generate foregut derivatives, hPSCs were first induced into DE as previously described (Fig. 1), followed by three-dimensional (3D) SOX2-expressing foregut spheroids (D'Amour et al., 2005; Dye et al., 2016); M...
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