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Preparation method and application of Phellinus linteus extract

A Phellinus extract, Phellinus technology, applied in the field of traditional Chinese medicine

Pending Publication Date: 2020-08-28
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Drugs with efficacy in colorectal cancer still to be studied

Method used

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  • Preparation method and application of Phellinus linteus extract
  • Preparation method and application of Phellinus linteus extract
  • Preparation method and application of Phellinus linteus extract

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Experimental program
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Effect test

Embodiment Construction

[0012] The following embodiments can enable those skilled in the art to understand the present disclosure more fully, but do not limit the present disclosure in any way.

[0013] In this experiment, the inhibitory effects of water extract, 60% ethanol extract and 95% ethanol extract on the proliferation of colon cancer cell SW480 cultured in vitro were compared to explore the anti-tumor mechanism of the components in Phellinus.

[0014] Instruments and reagents

[0015] LC-20AT Shimadzu High Performance Liquid Chromatograph, T6 New Century Seiwai Visible Spectrophotometer.

[0016] Colon cancer cells SW480 were purchased from Cybekon Biotechnology Co., Ltd.; CCK8 reagent was purchased from Meilun Biotechnology, product number: MA0218-5; p-AKT and mTOR primary antibodies were purchased from abcam, p-AKT product number: ab38449, concentration: 1mg / mL, diluted 1:1000 when used, mTOR product number: ab2732, concentration: 1mg / mL, diluted 1:1000 when used; AKT product number: ab17...

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Abstract

The invention provides a preparation method and an application of Phellinus linteus extract. The preparation method of the Phellinus igniarius extract comprises the steps as follows: weighing Phellinus igniarius powder, adding 60% ethanol in 20-times volume, performing heating, keeping boiling, and performing reflux extracting for 1 h to obtain an extract solution; centrifuging the extract solution to obtain a supernatant, and performing drying under reduced pressure until a product is dry to obtain the Phellinus linteus extract. The 60% alcohol extract of Phellinus igniarius can better inhibit proliferation of the colon cancer cell SW480, can better induce apoptosis of the colon cancer cell SW480, and induce the colon cancer cell SW480 to form G2 / M arrest to different degrees, so that proliferation of cancer cells is inhibited.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to a preparation method and application of Phellinus Phellinus extract. Background technique [0002] Phellinus japonica, also known as mulberry, is a very precious perennial large medicinal fungus that parasitizes on the woody substrate of Morus L. plants. Once recorded in the "Compendium of Materia Medica" by Li Shizhen in the Ming Dynasty, it is good at treating blood metrorrhagia, bloody stranguria, prolapse of the anus, diarrhea, leukorrhea, amenorrhea and other diseases. At present, Phellinus cultivars are used in China, Japan, South Korea and some European countries, and they are mostly used to prevent and treat various cancers. Because colorectal cancer (Colorectal cancer, CRC) is one of the malignant tumors with high incidence in the world, because of its high mortality rate, it has seriously endangered human health. The causes of colon cancer are complex and c...

Claims

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Application Information

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IPC IPC(8): A61K36/07A61P35/00
CPCA61K36/07A61K2236/333A61K2236/51A61K2236/53A61P35/00
Inventor 李娆娆郭珊珊
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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