Coposition containing chlopidogrel and antithrombotic agent active component
Technology of an active ingredient, clopidogrel, in the field of compositions containing active ingredients of clopidogrel and an antithrombotic agent
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[0036] The combination of active ingredients according to the invention is the subject of pharmaceutical research. As mentioned above, studies were conducted on experiments for agglutination of rabbit platelets with collagen (Born et al., J. Physiol., 1963, 168, 178-95). Briefly, 2.5-3 kg of New Zealand rabbits were treated for 3 days with ticlopidine (100 mg / kg / day) by the oral route or with clopidogrel (10 mg / kg) by the intravenous route. One hour after the last dose, animals were treated with aspirin (1 mg / kg) by intravenous route.
[0037] Five minutes after aspirin administration, the animal was anesthetized with ether, and a 2 ml blood sample was taken from the middle ear artery and mixed with 0.2 ml of 3.8% sodium citrate aqueous solution. Blood samples were centrifuged at 500g for 10 minutes at 15°C to obtain platelet-rich plasma. The number of platelets was then adjusted to 106 cells / [mu]l with the aid of platelet-depleted plasma obtained by centrifugation of antico...
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