Preparation method and application of ganoderma lucidum mycelium polysaccharide

A technology of ganoderma lucidum mycelium and polysaccharides, applied in the fields of application, polysaccharide/gum-containing food ingredients, medical preparations containing active ingredients, etc., can solve the problems of low purity of ganoderma lucidum polysaccharides, high extraction cost, waste of raw materials, etc., and achieve comprehensive Good utilization effect, simplified process steps, and improved extraction rate

Inactive Publication Date: 2020-09-04
广州颜如玉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this extraction method can indeed obtain a certain amount of Ganoderma lucidum polysaccharides, the purity of Ganoderma lucidum polysaccharides is not high, and it uses Ganoderma lucidum as the raw material for extraction, which causes a lot of waste of raw materials in the extraction p

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  • Preparation method and application of ganoderma lucidum mycelium polysaccharide
  • Preparation method and application of ganoderma lucidum mycelium polysaccharide

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Example Embodiment

[0029] Example 1. A kind of Ganoderma lucidum mycelium polysaccharide

[0030] The preparation method of Ganoderma lucidum mycelium polysaccharide comprises the following steps:

[0031] S1. Take the dried Ganoderma lucidum mycelium into a pulverizer and pulverize to 8μm, and then add 8 times the amount of organic solvent for degreasing treatment. The organic solvent consists of propylene glycol methyl ether, acetone and dimethyl sulfoxide in a mass ratio of 2: 4:1 composition, degreasing treatment time is 1h, centrifugal separation, take the lower layer of sediment and dry it at 50 ℃, to obtain Ganoderma lucidum mycelium coarse powder;

[0032] S2. Take the Ganoderma lucidum mycelium coarse powder prepared in step S1, add 10 times the amount of deionized water, and then perform supercritical CO 2 Extraction, supercritical CO 2 The extraction conditions are: pressure 30Mpa, extraction temperature 50℃, extraction time 4h; filter to prepare water extract;

[0033] S3. Take the water ext...

Example Embodiment

[0036] Example 2. A kind of Ganoderma lucidum mycelium polysaccharide

[0037] The preparation method of Ganoderma lucidum mycelium polysaccharide comprises the following steps:

[0038] S1. Take the dried Ganoderma lucidum mycelium into a pulverizer and pulverize to 8.5μm, and then add 8.5 times the amount of organic solvent for degreasing. The organic solvent consists of propylene glycol methyl ether, acetone and dimethyl sulfoxide in a mass ratio of 3 : 5:2 composition, degreasing treatment time is 1.5h, centrifugal separation, take the lower layer of sediment and dry it at 55°C to obtain Ganoderma lucidum mycelium coarse powder;

[0039] S2. Take the Ganoderma lucidum mycelium coarse powder prepared in step S1, add 14 times the amount of deionized water, and then perform supercritical CO 2 Extraction, supercritical CO 2 The extraction conditions are: pressure of 31Mpa, extraction temperature of 53°C, extraction time of 4.2h; filtration to prepare water extract;

[0040] S3. Take t...

Example Embodiment

[0043] Example 3. A kind of Ganoderma lucidum mycelium polysaccharide

[0044] The preparation method of Ganoderma lucidum mycelium polysaccharide comprises the following steps:

[0045] S1. Take the dried Ganoderma lucidum mycelium into a pulverizer and pulverize to 9μm, and then add 9 times the amount of organic solvent for degreasing. The organic solvent consists of propylene glycol methyl ether, acetone and dimethyl sulfoxide in a mass ratio of 2: 6:1 composition, degreasing treatment time of 2h, centrifugal separation, take the lower layer of sediment and dry it at 58°C to obtain the Ganoderma lucidum mycelium coarse powder;

[0046] S2. Take the Ganoderma lucidum mycelium coarse powder prepared in step S1, add 16 times the amount of deionized water, and then perform supercritical CO 2 Extraction, supercritical CO 2 The extraction conditions are: pressure 33Mpa, extraction temperature 55°C, extraction time 4.5h; filter to prepare water extract;

[0047] S3. Take the water extract...

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Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to ganoderma lucidum mycelium polysaccharide as well as a preparation method and application thereof. Ganoderma lucidum mycelia rich in ganoderma lucidum polysaccharides are adopted as an extraction raw material, and the ganoderma lucidum mycelia polysaccharides are prepared through the process steps of rawmaterial pretreatment, degreasing treatment, supercritical CO2 extraction, enzymolysis, alcohol precipitation and the like. The extraction rate of the ganoderma lucidum mycelium polysaccharides prepared by the preparation method of the ganoderma lucidum mycelium polysaccharides reaches 15.4% or above, and the purity of the ganoderma lucidum mycelium polysaccharides reaches 76.1% or above. The ganoderma lucidum mycelium polysaccharide is extracted by adopting a supercritical CO2 extraction technology and an enzymolysis technology, so that the extraction rate of the polysaccharide is increased,the waste of raw materials is avoided, the production cost is saved, and the prepared ganoderma lucidum mycelium polysaccharide is applied to preparation of health care products and medicines, has good effects, and is remarkable in economic benefit and social benefit.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a ganoderma lucidum mycelia polysaccharide and a preparation method and application thereof. Background technique [0002] Ganoderma lucidum mycelium is the mycelium formed by the germination of Ganoderma lucidum spores, and the constituent substances required by the fruiting body of Ganoderma lucidum. More than half of the nutrients come from the nutrients stored in the weight of the mycelium. The content of Ganoderma lucidum is generally many times higher than that of Ganoderma lucidum fruiting body and Ganoderma lucidum spore powder. [0003] Ganoderma lucidum mycelium polysaccharide is composed of three strands of monosaccharide chains, and its configuration is similar to DNA and RNA. It is a helical three-dimensional configuration, and the helical layers are mainly fixed by hydrogen bonds. The polysaccharides of Ganoderma lucidum mycelium have significa...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P37/02A61P35/00A61P39/06A61P9/00A61P9/12A61P11/14A61P37/08A23L33/125
CPCA23V2002/00A61K31/715A23L33/125A61P9/00A61P9/12A61P11/14A61P35/00A61P37/02A61P37/08A61P39/06C08B37/0003A23V2200/302A23V2200/304A23V2200/314A23V2200/308A23V2200/324A23V2200/326A23V2250/51Y02P20/54
Inventor 王中振谢涛
Owner 广州颜如玉生物科技有限公司
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