Focusing method and system

Abstract

The invention belongs to the technical field of focusing, and relates to a focusing method and system. The method comprises: carrying out image definition evaluation and target content evaluation on asample image acquired by an image acquisition system; continuously evaluating an image view corresponding to the target content evaluation score higher than a threshold value; and for the purpose offinding the peak of the image definition evaluation curve, determining next sampling displacement according to the trend of the image definition curve, repeating image acquisition and evaluation untilthe peak of the image focusing curve under each visual field is found, and determining the focusing position under the current focusing precision under each visual field according to the sampling displacement corresponding to the peak. According to the focusing method, rapid focusing can be realized under the condition of ensuring a relatively large search range, relatively high focusing accuracyand relatively high focusing position accuracy.

Description

technical field [0001] The present invention belongs to the technical field of focusing, and more specifically relates to a focusing method and system. Background technique [0002] Hospital pathology departments mainly use biological slides made from patient tissues for diagnosis. Due to the needs of informatization, digitization, and intelligence in the department, it is necessary to convert biological slides into digital images, that is, digital scanning. figure 2 It is a schematic diagram of the traditional pathological slide structure and its relationship with the objective lens, where: [0003] A: Glass slide is the carrier of tissue samples. The commonly used glass slide size is 76mm (length) X 26mm (width), and the thickness is 1.1±0.04mm; [0004] B: Tissue sample, a thin slice of tissue removed from the sample during film production. For conventional pathological tissue slides, use a microtome to manually cut tissue samples from the tissue block, and the thickne...

AI Technical Summary

Problems solved by technology

[0024] In view of the above defects or improvement needs of the prior art, the present invention provides a focusing method and system, which uses the search control method, based on the existing displacement platform and image acquisition system, to monitor the sample on the displacement platform from any position Carry out sampling and evaluate the sampling results, control the next sampling position according to the trend of the curve formed by the evaluation results, and continue this process until the focus position is searched at the minimum step, thereby solving the problem of the number of sampling times of the focus method in the prior art Many, slow focusing speed, small search range, poor adaptability and other technical problems

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