A Lactobacillus plantarum capable of scavenging acrylamide and its application
A technology of Lactobacillus plantarum and acrylamide, applied in the field of microorganisms, can solve problems such as easy polymerization, and achieve the effect of great application prospects
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Embodiment 1
[0049] Example 1: Acquisition of Lactobacillus plantarum CCFM1124
[0050] Specific steps are as follows:
[0051] 1. Separation and purification
[0052] (1) Dilution coating: Take the intestinal contents of people from Changji military household farm in Xinjiang as a sample, draw 0.5g of the sample preserved in 30% (v / v) glycerin, add 4.5 mL of normal saline in a 10mL centrifuge tube to obtain 10 -1 Diluent, repeat the above dilution steps to obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Diluent;
[0053] (2) Coating culture: draw 100 μL of the 10 cells obtained in step (1) respectively -4 、10 -5 、10 -6 The three gradient dilutions were coated on the LFMATA solid medium, and cultured at 37°C for 48 hours to obtain a diluted coating plate;
[0054] (3) Primary purification culture: take the diluted coating plate with the number of colonies obtained in step (2) ranging from 30 to 300, randomly select 10 milky white or white ones on each diluted coating plate, with smooth...
Embodiment 2
[0063] Embodiment 2: the scavenging action of acrylamide by Lactobacillus plantarum CCFM1124
[0064] Specific steps are as follows:
[0065] Accurately weigh 10 mg of acrylamide standard product and dissolve it in 1 mL of sterile water, mix well and dissolve, then use a 0.22 μm microporous filter membrane to filter and sterilize to obtain acrylamide standard product mother solution with a concentration of 10 mg / mL; accurately weigh 10 mg 100 μL of acrylamide standard stock solution per mL was dilute to 100 mL with sterile water to obtain acrylamide standard stock solution with a concentration of 10 μg / mL; The mother liquor was used as a blank control, and 3×10 9 CFU of Lactobacillus plantarum QS6-12 cells, Lactobacillus plantarum RS15-3 cells, Lactobacillus plantarum CCFM1124 cells, Lactobacillus plantarum VJLHD7-L1 cells, Lactobacillus plantarum VJLHD7-L1 cells, Lactobacillus plantarum VJLHD11- L1 cells, Lactobacillus plantarum FJHLD57M1 cells, Lactobacillus plantarum QHLJ...
Embodiment 3
[0068] Embodiment 3: the application of Lactobacillus plantarum CCFM1124
[0069] Specific steps are as follows:
[0070] Lactobacillus plantarum CCFM1124 can be used to prepare bacterial powder, and the specific preparation process of bacterial powder is as follows:
[0071] Lactobacillus plantarum CCFM1124 was streaked on the MRS solid medium and cultured at 37°C for 48 hours to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, culture it at 37°C for 18 hours for activation, and continuously activate two generations. Obtain the activated solution; inoculate the activated solution in the culture medium with an inoculum size of 2% (v / v), cultivate it at 37°C for 18 hours to obtain the bacterial solution; centrifuge the bacterial solution at 5000g for 15 minutes to obtain the bacterial sludge; After washing 3 times with normal saline, resuspend with protective agent to a concentration of 1×10 10 CFU / mL to obtain a bacterial suspension; pr...
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