A long-term preservation method of Cordyceps militaris strain with high cordycepin production capacity

Abstract

The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a method for long-term preservation of Cordyceps militaris strains with high production capacity of cordycepin. Inoculate the strain of Cordyceps militaris on the slant medium of the test tube, cultivate it in a constant temperature incubator at 18-25°C, wait until the mycelium grows all over the slope, and obtain the slant strain; the slant strain is cut into pieces under a sterile environment to obtain the mycelium-containing Bacterial blocks; add protective solution and bacterial blocks containing mycelia to the cryopreservation tube, and completely submerge the bacterial blocks in the protective solution, pre-preserve the cryopreservation tubes with a variable temperature treatment method, and then take the cryopreservation tubes for inspection, select The surviving strains were stored at -80°C for a long time. The invention adopts the culture medium containing ginkgo fruit powder, which can maintain the cordycepin-producing activity of the Cordyceps militaris strain. The present invention also adopts a variable temperature treatment method of "lowering the room temperature to -10°C, then raising the temperature to 4°C, and finally dropping to -80°C" to improve the anti-low temperature performance of the strain and retain the activity of the Cordyceps militaris strain.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a method for long-term preservation of Cordyceps militaris strains with high production capacity of cordycepin. Background technique [0002] Cordyceps militaris belongs to the Ascomycota subphylum Ergotaceae Cordyceps genus, is an important traditional Chinese medicine. Cordycepin contained in Cordyceps militaris is a nucleoside antibiotic, which has antibacterial, anti-inflammatory, antiviral, and antitumor activities, and has high nutritional and medicinal value. The screening and preservation of Cordycepin militaris strains with high production capacity of cordycepin is an important prerequisite for the separation and extraction of cordycepin. The number of Cordyceps militaris grown in nature is small and the yield is low, so the technology of artificially cultivating high-cordycepin Cordyceps militaris is gradually rising. [0003] In the pr...

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Problems solved by technology

[0003] In the prior art, the preservation methods of the Cordyceps militaris bacterial classification mainly include 4-15 ℃ test tube slant refrigeration, 26 ℃ air-conditioned room preservation, 4-15 ℃ test tube slant plus paraffin oil refrigeration, 26 ℃ test tube slant plus sterile water air conditioning Preservation and other methods, among them, 26°C air-conditioned room preservation and 26°C test tube slope plus sterile water air-conditioning method are suitable for short-term preservation, which can effectively guarantee the activity of contemporary strains and can be used as they are taken, but the strains reproduce at this temperature Fast, not conducive to the preservation of offspring strains

4-15°C test tube slant storage is an important method for long-term preservation of Cordyceps militaris strains due to the low temperature and the bacteria can survive for a long time. However, the activity of the bacteria will decrease after long-term storage on the slope, and rejuvenation is often required. Some The rejuvenation of the strain after long-term refrigeration is unsuccessful, and there is a risk of reduced production activity of cordycepin, resulting in a decrease in the production of cordycepin

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