Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for constructing an animal model of allergic rhinitis with syndrome of lung-Qi deficiency syndrome combined with disease and syndrome

A technology for allergic rhinitis and animal models, applied in animal husbandry and other directions, can solve the problems of high cost, different concentrations of senna leaves, inconsistent thinking of syndrome modeling and disease modeling, etc., and achieves low mortality and high price. cheap effect

Active Publication Date: 2022-02-18
GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
View PDF15 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many scholars in China have constructed some syndrome-related animal models using different approaches and ideas, including AR animal models such as lung-qi deficiency and spleen-qi deficiency. Modeling and disease modeling ideas are not unified
The fumigation method is currently the most widely used modeling method in lung-qi deficiency syndrome experiments, but the fumigation equipment, fumigation time, and fumigation volume are different. The existing fumigation equipment on the market is expensive and is not equipped in general laboratories. ; and the smoke concentration, temperature and ventilation treatment in the self-made smoking box are the key issues in the preparation of the AR model
Using senna cold extract to gavage is a simple and easy way to make Qi deficiency, but the concentration of senna used in the prior art is different

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for constructing an animal model of allergic rhinitis with syndrome of lung-Qi deficiency syndrome combined with disease and syndrome
  • A method for constructing an animal model of allergic rhinitis with syndrome of lung-Qi deficiency syndrome combined with disease and syndrome
  • A method for constructing an animal model of allergic rhinitis with syndrome of lung-Qi deficiency syndrome combined with disease and syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This embodiment provides a smoking box for constructing a combination animal model of lung-Qi deficiency and allergic rhinitis, including a storage box (1) and a smoking accessory (3); the smoking accessory includes a The column (32) and the base (33) of the chimney (31); the chimney (31) is positioned at the top of the column (32) and communicates with the top; the bottom of the column is open, not closed, and The side of the cylinder near the bottom is provided with ventilation holes (34); the top of the base (33) is open and the bottom is closed, and the cylinder (32) is nested on the base (33) ( Figure 12 ).

[0038] The smoking accessories in this embodiment are made of stainless steel; the cylinder and the base are cylindrical; the ventilation holes on the side of the cylinder are square; the storage box is made of plastic and has a size of 60 cm (length). × 40cm (wide) × 35cm (high), the diameter of the cylindrical stainless steel cylinder is 7cm, and the heigh...

Embodiment 2

[0040] Example 2 Constructing an animal model of combined diseases and syndromes of allergic rhinitis with lung-Qi deficiency syndrome

[0041] Step 1: Combining smoking and gavage with senna leaves to construct rats with complex syndrome of lung-Qi deficiency, the specific steps are as follows:

[0042] 1) After the SD rats were quarantined for 3 days, they were placed in a mouse cage and then placed in a self-made fume box. Smoke 8 cigarettes at a time for 20 minutes, then ventilate and rest for 10 minutes, and smoke continuously for 3 times. The rats are smoked for 1 hour every day. 24 cigarettes are used in total, and the smoking modeling period is 60 days in total.

[0043] 2) Start gavage from the 31st day of smoking, put senna leaves into boiling water and soak for 1 hour (the ratio of 1g (0.1g / ml) senna leaves in 10ml water), filter out the liquid medicine and store at 4°C Refrigerator for spare. Rats were administered orally at 1ml / 100g body weight, once a day. The c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for constructing an animal model of combined lung-Qi deficiency and allergic rhinitis syndrome, comprising the following steps: constructing an animal model of lung-Qi deficiency combined syndrome by combining smoking and gavage with senna leaves, Then the animal model of allergic rhinitis was established by intraperitoneal injection of ovalbumin and aluminum hydroxide suspension and intranasal instillation of ovalbumin. The construction method of the present invention is based on the theory of traditional Chinese medicine that internal and external factors cause disease at the same time, coordinates multi-factor joint modeling, combines physical and chemical factors, and combines diseases and syndromes to establish a compound syndrome disease animal model. Through repeated experimental evaluation, various modeling factors are quantified, the modeling time course is concrete, and the animals have the advantages of standardization and high reproducibility. In addition, the self-made smoking equipment in the present invention is simple and easy to replicate, and the price is cheap, and the transparent plastic storage box and self-made stainless steel small accessories on the market can be used.

Description

technical field [0001] The invention relates to the technical field of animal models, in particular to a method for constructing an animal model combined with symptoms of lung-qi deficiency and allergic rhinitis. Background technique [0002] Allergic rhinitis (Allergic Rhinitis, AR) is a global disease that affects health, and its incidence rate is as high as 40%, and it is increasing year by year. Traditional Chinese medicine believes that the occurrence of AR is based on the deficiency of the lungs, spleen, and kidneys, and is caused by the feeling of wind and cold and abnormal qi, and the nose orifices are affected by evil. Modern literature reports that deficiency of lung and spleen is a common clinical syndrome of AR. Physicians in ancient and modern times all believe that although AR is a disease of the lung orifices, it is most closely related to spleen deficiency. For example, it is recorded in "Su Wen" that "the spleen is an isolated organ... if it is not as good ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02A01K2207/20A01K2207/35A01K2227/105A01K2267/0368
Inventor 罗秋兰黄唯冯小聪周世卿刘华娜谭希陈海李云英
Owner GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE