Method for improving quality of aquaculture pond water using a nutrient germinant composition and spore incubation method

An aquaculture, composition technology, applied in chemical instruments and methods, biological water/sewage treatment, polluted waterway/lake/pond/river treatment, etc., can solve problems such as deterioration, low nitrification activity, increased disease pressure, etc. , to achieve the effect of improving yield, promoting nitrification activity and ammonia reduction, and reducing disease pressure

Pending Publication Date: 2020-10-13
NCH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Issues associated with this process include: pollution discharge from feedlots that will deteriorate surrounding water quality; product loss due to degraded water quality in feedlots; increased disease pressure associated with pathogenic microorganisms in feedlots
Issues associated with current technologies include: high cost, poor water quality improvement performance due to inactive supplementary bacteria, low nitrification activity due to presence of organic waste and lack of growth sites for nitrifying bacteria, and bioaccumulation of antibiotics in farmed aquatic species
For example, B. licheniformis (B. licheniformis) and B. subtilis (B. subtilis) spores will germinate at 35°C or 37°C, respectively, but they take longer in germination buffers containing vegetative germination agents (e.g. ,2 hours)

Method used

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  • Method for improving quality of aquaculture pond water using a nutrient germinant composition and spore incubation method
  • Method for improving quality of aquaculture pond water using a nutrient germinant composition and spore incubation method
  • Method for improving quality of aquaculture pond water using a nutrient germinant composition and spore incubation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] Example 1 - In order to germinate the spores, FreeFlow LF-88 probiotics (commercially available spore liquid preparation from NCH Company) were added to a final concentration of about 1×10 9 CFU / mL was added to 1 mL of tap water, where CFU stands for colony forming unit. A preferred embodiment according to the invention comprising L-alanine (89 g / L), sodium dihydrogen phosphate (20 g / L), disodium hydrogen phosphate (60 g / L) and Linguard CP (total 1.6 g / L) The nutrient germinant concentrate composition was added to the water and bacteria mixture to provide a 4% final concentration of the nutrient germinant composition based on the total weight of the mixture. For comparison, a negative control reaction was prepared with the same amount of free-flow LF-88 probiotic bacteria and water, but without the addition of the nutrient germination concentrate composition. The two mixtures (germinant without nutrient germinant composition and negative control) were blended and incub...

Embodiment 2

[0118] Example 2 - Another set of incubation tests was performed using the same test mixture / incubation method (incubation using the same nutrient germination composition and heat, "treated spores, 42°C") and repeating what was done in Example 1 above Control mixture / incubation method as described (no nutrient germination composition and no heat, untreated spores, 23° C.), but a different test was performed to compare the performance of the test mixture according to a preferred embodiment of the invention compared to the control mixture. effect. In addition, two other mixtures were tested - one of which used the vegetative germination composition of Example 1 but without heating ("Treated spores, 23°C"), and the other without the vegetative germination agent but with spores heated ("Untreated spores, 42°C"). Briefly, spores were incubated at 42°C or 23°C for 1 hour with or without treatment with the preferred vegetative germination composition. After incubation, spores from ...

Embodiment 3

[0123] Example 3 - Another set of incubation tests was performed using similar test and control mixtures and incubation methods as described in Example 1 above. In short, the LF-88 with about 10 8 A final concentration of CFU / mL was added to 10 mL of distilled water. Samples were incubated at various temperatures to demonstrate the efficacy of the test method according to the preferred embodiment of the present invention compared to a control mixture. Prepare the reaction with the vegetative germination composition described in Example 1 ( Figure 9 "Treated spores" in ) and incubated at 23°C (ambient temperature, no heating), 32°C, 42°C or 60°C. Control reactions were incubated at ambient room temperature without the nutrient germination composition. After 1 h incubation, 1 mL of each reaction was pelleted at 14K RPM for 3 min at 23 °C and resuspended in Butterfield buffer. Will be about .6x10 5 CFU (0.02 mL) was added to 0.980 mL of Davis minimal medium (containing 3% g...

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Abstract

A method for improving the quality of pond water used in aquaculture applications by adding to the pond water active bacteria that are preferably germinated from spores on site using a nutrient-germinant composition and an incubation method for increased spore germination efficiency, in combination with a nitrification enhancement agent such as calcium carbonate or calcified seaweed, and an optional reaction surface area modifier such as calcified seaweed or plastic or metal particles or fragments. The nutrient-germinant composition comprises L-amino acids, D-glucose and/ or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores (preferably of one or more Bacillus species) or they may be separately combined for germination. The incubation method comprises heating a nutrient germinant composition and bacteria spores, to a temperature range of 35 DEG C to 60 DEG C for around 2 to 60 minutes to produce an incubated bacteria solution that is dischargedto the aquaculture application.

Description

[0001] Citations to related applications [0002] This application claims the benefit of U.S. Application Serial No. 15 / 907,682, filed February 28, 2018. technical field [0003] The present invention relates to the treatment of aquaculture pond water with bacteria germinated in vegetative germinative compositions and point-of-use spore hatching to reduce organic waste, ammonia and disease stress in aquatic animal husbandry applications and to provide probiotics to aquaculture species . Background technique [0004] Aquaculture refers to the farming of aquatic species used as a food source for humans or animals. The technology applies certain types of controls to the natural environment in which species are reared to improve overall yield. This may include hatching species to augment commercial harvests of animals in the wild, hatching and rearing species in enclosed ponds, and hatching and rearing species in tidal drained enclosures close to shorelines. Issues associated...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/13A01K63/04C02F3/34
CPCC02F2209/02C02F3/341C02F2103/20C02F3/303C02F3/308C02F3/302C02F3/348C02F2103/007C12N1/20
Inventor 查尔斯·J·格林沃尔德加布里埃尔·F·K·埃弗里特朱迪·普鲁伊特阿曼达·罗斯马里恩约尔丹·E·丘奇丹尼尔·阿伯勒乔治·阿博阿吉耶斯凯拉·雷·怀特曹海波克里斯托弗·泽特纳凯利·吉莱斯皮
Owner NCH CORP
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