Evaluation method of CircRNA as liver cancer tumor marker

A tumor marker and evaluation method technology, which is applied in the field of evaluation of circRNA as a tumor marker for liver cancer, and achieves the effects of simple operation method, improved cell activity, and improved extraction efficiency.

Pending Publication Date: 2020-10-16
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how to use RNA to detect target

Method used

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  • Evaluation method of CircRNA as liver cancer tumor marker
  • Evaluation method of CircRNA as liver cancer tumor marker

Examples

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Effect test

Embodiment 1

[0039] Example 1 A method for evaluating circRNA as a liver cancer tumor marker

[0040] The CircRNA is used as an evaluation method for liver cancer tumor markers, including the following processes:

[0041] S1. Take the CD90posi cells in the liver cancer tissue, carry out primary and subculture, and obtain the CD90posi cells;

[0042] Among them, the primary culture adopts tissue block culture, and the specific operation process is as follows:

[0043] (1) Take liver cancer tissue, disinfect it with 75% ethanol, place it in a sterile petri dish, wash it with Hanks solution for 3 times, cut the tissue as much as possible with elbow scissors, each tissue piece is less than 1mm 3 , get the organization block of preliminary processing;

[0044] (2) The preliminary treated tissue pieces prepared in step (1) were washed twice with Hanks solution, centrifuged for 10 min at a speed of 50 rpm, removed the supernatant, and then added penicillin and streptomycin in a mass ratio of 3 ...

Embodiment 2

[0053] Example 2 A method for evaluating circRNA as a liver cancer tumor marker

[0054] The CircRNA is used as an evaluation method for liver cancer tumor markers, including the following process:

[0055] S1. Take the CD90posi cells in the liver cancer tissue, carry out primary and subculture, and obtain the CD90posi cells;

[0056] Among them, the primary culture adopts tissue block culture, and the specific operation process is as follows:

[0057] (1) Take liver cancer tissue, disinfect it with 75% ethanol, place it in a sterile petri dish, wash it with Hanks solution for 3 times, cut the tissue as much as possible with elbow scissors, each tissue piece is less than 1mm 3 , get the organization block of preliminary processing;

[0058] (2) The preliminary treated tissue pieces prepared in step (1) were washed 5 times with Hanks solution, centrifuged for 10 min at a rotating speed of 50 rpm, removed the supernatant, and then added penicillin and streptomycin in a mass ra...

Embodiment 3

[0067] Example 3 A method for evaluating circRNA as a liver cancer tumor marker

[0068] The CircRNA is used as an evaluation method for liver cancer tumor markers, including the following processes:

[0069] S1. Take the CD90posi cells in the liver cancer tissue, carry out primary and subculture, and obtain the CD90posi cells;

[0070] Among them, the primary culture adopts tissue block culture, and the specific operation process is as follows:

[0071] (1) Take liver cancer tissue, disinfect it with 75% ethanol, place it in a sterile petri dish, wash it with Hanks solution for 3 times, cut the tissue as much as possible with elbow scissors, each tissue piece is less than 1mm 3 , get the organization block of preliminary processing;

[0072] (2) The preliminary treated tissue pieces prepared in step (1) were washed 4 times with Hanks solution, centrifuged for 10 min at a speed of 50 rpm, removed the supernatant, and then added penicillin and streptomycin in a mass ratio of ...

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Abstract

The invention belongs to the technical field of biotechnology and genetic engineering, and particularly relates to an evaluation method of CircRNA as a liver cancer tumor marker. According to the evaluation method provided by the invention, CircRNA of a CD 90 posi cell subset is used as the tumor marker, the number of tumor cells CD 90 posi in a liver cancer patient is detected, and then, the severity degree of the liver cancer is evaluated. The evaluation method provided by the invention has the advantages that an operation process is simple; the enrichment degree of the CD 90 posi cells canbe effectively detected; a novel technology is provided for tumor detection; and a novel idea is also provided for tumor treatment.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and genetic engineering, and specifically relates to a method for evaluating CircRNA as a liver cancer tumor marker. Background technique [0002] Primary liver cancer is one of the most common malignant tumors in my country. Its morbidity and mortality are high, and its case fatality rate ranks second in domestic tumors, which seriously threatens the life and health of the people. The onset of liver cancer is hidden, and the clinical manifestations are lacking in particularity. When seeking medical treatment, the patients are often in the middle and late stages of liver cancer. It is difficult to find patients with early liver cancer, which seriously affects the treatment effect of liver cancer. The latest clinical statistics show that about 800,000 new primary liver cancer patients are diagnosed in China every year, accounting for 55% of the global total; the annual death rate due to prima...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113C12N5/09C12N5/071
CPCC12Q1/6886C12N5/0693C12N5/067C12Q2600/178C12Q2600/158C12N2500/76C12N2501/998
Inventor 刘建平刘尊龙任飞
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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