Test strip, kit and detection method for detecting novel coronavirus (2019-nCoV) antigen

A 2019-ncov, coronavirus technology, applied in the field of human respiratory virus detection, can solve the problems of high technical personnel requirements, high experimental operation requirements, complex operations, etc., to achieve the effect of strong operability, improved sensitivity, and simple operation

Pending Publication Date: 2020-10-30
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the gold standard for clinical virus detection is still the virus culture method, but this method takes a long time, has low sensitivity, and requires high experimental operations.
The current detection method for th

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: A test strip for detecting novel coronavirus (2019-nCoV) antigens, including a colloidal gold double conjugate pad sample pad, the colloidal gold double conjugate pad comprising a streptavidin conjugate pad and a double Label the antibody conjugate pad, in addition to the nitrocellulose membrane, absorbent paper, and bottom plate.

[0043] The preparation method of the streptavidin conjugate pad is as follows:

[0044] Adjust the pH of the 40nm colloidal gold solution to 9.0, add streptavidin to a final concentration of 17 μg / mL, and let stand at room temperature for 1 h. Add 1 / 10 volume of phosphate solution containing 10% BSA, let it stand at room temperature for 30 minutes, centrifuge at 4°C and 7000 rpm for 30 minutes, the precipitate obtained is the colloidal gold-labeled streptavidin, and the colloidal gold labeling substance is obtained .

[0045]Take the glass fiber and put it into a stainless steel plate, measure 45mL of the colloidal gold marker a...

Embodiment 2

[0055] Example 2: A test strip for detecting novel coronavirus (2019-nCoV) antigens, including colloidal gold double conjugate pad sample pad, said colloidal gold double conjugate pad including streptavidin conjugate pad and double Label the antibody conjugate pad, in addition to the nitrocellulose membrane, absorbent paper, and bottom plate.

[0056] The preparation method of the streptavidin conjugate pad is as follows:

[0057] Adjust the pH of the 40nm colloidal gold solution to 9.0, add streptavidin to a final concentration of 15 μg / mL, and let stand at room temperature for 1 h. Add 1 / 10 volume of phosphate solution containing 10% BSA, let it stand at room temperature for 30 minutes, centrifuge at 4°C and 7000 rpm for 30 minutes, the precipitate obtained is the colloidal gold-labeled streptavidin, and the colloidal gold labeling substance is obtained .

[0058] Take the glass fiber and put it into a stainless steel plate, measure 45mL of the colloidal gold marker after ...

Embodiment 3

[0068] Example 3: A test strip for detecting novel coronavirus (2019-nCoV) antigens, including colloidal gold double conjugate pad sample pad, said colloidal gold double conjugate pad including streptavidin conjugate pad and double Label the antibody conjugate pad, in addition to the nitrocellulose membrane, absorbent paper, and bottom plate.

[0069] The preparation method of the streptavidin conjugate pad is as follows:

[0070] Adjust the pH of the 40nm colloidal gold solution to 9.0, add streptavidin to a final concentration of 19 μg / mL, let stand at room temperature for 1 hour, add 1 / 10 volume of phosphate solution containing 10% BSA, stand at room temperature for 30 minutes, Centrifuge at 7,000 rpm for 30 min to obtain a precipitate that is colloidal gold-labeled streptavidin to prepare a colloidal gold-labeled substance.

[0071] Take the glass fiber and put it into a stainless steel plate, measure 40mL of the colloidal gold marker after reconstitution and pour it into...

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PUM

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Abstract

The invention relates to a test strip for detecting a novel coronavirus (2019-nCoV) antigen, the test strip comprises a colloidal gold double conjugate pad, the colloidal gold double conjugate pad comprises a streptavidin conjugate pad and a double-labeled antibody conjugate pad, and the double-labeled antibody conjugate pad is a colloidal gold and biotin label; a biotin-avidin amplification system is introduced when the colloidal gold double conjugate pad is prepared, and a double-labeled antibody is adopted, so that the antigen detection sensitivity is improved, and missing detection is effectively reduced.

Description

technical field [0001] The invention relates to the field of human respiratory virus detection, in particular to a test strip, a kit and a detection method for the detection of novel coronavirus (2019-nCoV) antigens. Background technique [0002] At present, infectious diseases are still a major threat to human health, especially acute respiratory infectious diseases, which have high morbidity and mortality due to their many types, rapid spread, and wide prevalence, and have caused great harm to human health, social stability, and economic development. threaten. Winter and spring are the seasons of high incidence of respiratory infectious diseases. The main pathogens of respiratory tract infections are viruses. Due to the frequent mutation of respiratory viruses, new viruses or mutant strains continue to appear, which often easily cause outbreaks of new / emergent infectious diseases. [0003] 2019-nCoV has a long incubation period, generally 3-7 days, and the longest is no...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/569G01N33/558
CPCG01N33/583G01N33/56983G01N33/558G01N2469/10G01N2333/165
Inventor 杨致亭王传新冯照雷杜鲁涛李娟刘仁萍杨明霞
Owner 山东康华生物医疗科技股份有限公司
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