Preparation method of three-dimensional porous graphene film and micro-fluidic chip thereof

Abstract

The invention relates to the technical field of micro-fluidic chips, and particularly discloses a preparation method of a three-dimensional porous graphene film and a micro-fluidic chip thereof. The preparation method comprises the following steps: firstly, preparing graphene oxide hydrosol, adding the graphene oxide hydrosol, a photosensitive acrylate matrix and a photoinitiator into the grapheneoxide hydrosol subjected to ultrasonic treatment, carrying out ultrasonic treatment again, and preparing a required preparation body with a solid three-dimensional structure by adopting a projectionmicro-stereolithography technology, wherein the graphene oxide is trapped in a rigid long chain of an acrylic polymer. In the invention, the three-dimensional porous graphene film is manufactured by adopting a projection micro-stereolithography technology, two-dimensional graphene nanosheets can be assembled in a large range, original physicochemical properties of the graphene nanosheets are reserved while the graphene nanosheets form a three-dimensional structure, and the graphene nanosheets have a large specific surface area, a large three-dimensional conductive path and a large porous structure; meanwhile, more active sites can be provided for immobilization of immune protein, capture and immobilization of biological particles are facilitated, and tumor biomarkers can be remarkably detected.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a method for preparing a three-dimensional porous graphene film and a microfluidic chip. Background technique [0002] Microfluidic Chip (Microfluidic Chip) refers to a micro-dose fluid (10 -9 -10 -18 L) Excellent technique for performing various manipulation experiments. [0003] Electrochemical detection is a commonly used analytical detection method. Its principle is to use electrodes as sensors to directly convert the chemical signals of the components to be measured in the solution into electrical signals. [0004] An electrochemical microfluidic detection chip can be obtained by integrating the electrodes for electrochemical detection on the microfluidic chip. In the field of bioanalysis, people use chips to capture tumor cell-related protein markers. The detection requires less sample volume and low detection limit, which highlights good application value in ...

AI Technical Summary

Problems solved by technology

[0007] Aiming at the technical problem that the existing three-dimensional porous graphene layer is difficult to construct a controllable three-dimensional structure, and the three-dimensional structure is too simple and simple, the present invention provides a preparation method of a three-dimensional porous graphene film and its microfluidic chip, which can Scope of assembly of two-dimensional graphene nanosheets, which retains its original physical and chemical properties while forming a three-dimensional structure, with large specific surface area, three-dimensional conductive paths and porous structure

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