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Marasmius androsaceus exopolysaccharide and application thereof

A technology of Anluo Xiaopi umbrella and exopolysaccharide, which is applied in the field of Anluo Xiaopi umbrella exopolysaccharide and its application, can solve the problems that hinder the medicinal level of Anluo Xiaopi umbrella, complex components, etc., and achieve novel structure, The effect of various particle shapes and uniform components

Pending Publication Date: 2020-11-17
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current research mainly focuses on the crude polysaccharide components of Anluo Xiaopijiao, and the composition of crude polysaccharides is complex, which hinders the improvement of the medicinal level of Anluo Xiaopijiao. There are no reports on the research on the components and their related activities.

Method used

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  • Marasmius androsaceus exopolysaccharide and application thereof
  • Marasmius androsaceus exopolysaccharide and application thereof
  • Marasmius androsaceus exopolysaccharide and application thereof

Examples

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preparation example Construction

[0048] In one embodiment of the present invention, in step (1), the preparation method of the potato culture medium comprises: peeling 200-300g of potatoes, dicing, boiling in water for 30min, filtering, adding 15-20g of glucose, and distilling to 1L.

[0049] Further, in step (1), the activation conditions include: the activation is carried out in a shaker, the shaker speed is 180rpm-250rpm; the activation temperature is 26°C-30°C, and the activation time is 5-8 days.

[0050] In one embodiment of the present invention, in step (2), the medium for fermentation culture is: sucrose and / or glucose 10-20g / L, yeast extract powder and / or yeast extract and / or peptone 20-35g / L, KH 2 PO4 2.0-3.0g / L, MgSO 4 ·7H 2 O 2.0-3.5g / L, pH 7.0. Sucrose and / or glucose is at least one of sucrose or glucose. At least one of yeast extract powder and / or yeast extract and / or peptone.

[0051] Further, in step (2), the conditions for fermentation culture include: the rotation speed is 220rpm-300rp...

Embodiment 1

[0067] Example 1 Extraction and separation of polysaccharide MEPS3 from Anluo parsley polysaccharide

[0068] (1) Activation of strains: Anluo Xiaopi umbrella was inoculated in potato medium for activation to obtain seed liquid;

[0069] Among them, the preparation method of potato medium includes: peeling 250g of potatoes, dicing, boiling in water for 30min, filtering, adding 18g of glucose, and distilling to 1L; The time is 7 days.

[0070] (2) Strain cultivation: inoculate the seed liquid obtained in step (1) into a fermenter, cultivate, and obtain a fermented liquid;

[0071] Among them, the fermentation medium is: sucrose and / or glucose 15g / L, yeast extract powder and / or yeast extract and / or peptone 28g / L, KH 2 PO 4 2.5g / L, MgSO 4 ·7H 2 O 3g / L, pH 7.0; fermentation culture conditions: rotation speed 260rpm, fermentation temperature 28°C, fermentation time 7 days, ventilation rate 200L / h.

[0072] (3) Polysaccharide extraction: The fermented liquid obtained in ste...

Embodiment 2

[0075] Example 2 Extraction and separation of polysaccharide MEPS3 from Anluo parsley polysaccharide

[0076] (1) Activation of strains: Anluo Xiaopi umbrella was inoculated in potato medium for activation to obtain seed liquid;

[0077] Among them, the preparation method of potato culture medium includes: peeling 200g of potatoes, dicing, boiling in water for 30min, filtering, adding 15g of glucose, and distilling to 1L; The time is 5 days.

[0078] (2) Strain cultivation: inoculate the seed liquid obtained in step (1) into a fermenter, cultivate, and obtain a fermented liquid;

[0079] Among them, the fermentation medium is: sucrose 10g / L, yeast extract powder, yeast extract, peptone 20g / L, KH 2 PO 4 2.0g / L, MgSO 4 ·7H 2 O 2.0g / L, pH 7.0; fermentation culture conditions: rotation speed 220rpm, fermentation temperature 26°C, fermentation time 5 days, ventilation rate 150L / h.

[0080] (3) Polysaccharide extraction: The fermented liquid obtained in step (2) is filtered...

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Abstract

The invention provides marasmius androsaceus exopolysaccharide and an application thereof, and belongs to the technical field of biological extraction. The molecular weight of the marasmius androsaceus exopolysaccharide is 10.47 kDa, wherein the molar ratio of mannose to glucose to galactose in the monosaccharide composition is 0.08: 0.34: 1.46. The marasmius androsaceus exopolysaccharide is extracted from marasmius androsaceus fermentation liquor for the first time, is novel in structure, has a good anti-fatigue effect by adopting an animal experiment to test MEPS3 polysaccharide, and is mainly used for preparing anti-fatigue medicines, health care products and the like.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to an exopolysaccharide of the umbrella of Anluo and its application. Background technique [0002] Anluo small skin umbrella (Marasmius androsaceus) is a kind of medicinal fungus, which generally grows in dense woodland. Wait. Studies have shown that Anluo Xiaopi umbrella contains many active substances, such as cholesterol acetate, amino acids, ergosterol, cinnamic acid, β-sitosterol, alkaloids, proteins, mannitol, adenosine and polysaccharides and other macromolecular components. [0003] Among them, polysaccharides have a variety of biological activities and functions, such as anti-oxidation, lowering blood sugar, enhancing immunity, etc., and their pharmacological activities are closely related to their structures. At present, the pharmacological research on Anluo Xiaopi polysaccharide mainly focuses on its immune regulation, anti-oxidation and analg...

Claims

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Application Information

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IPC IPC(8): C12P19/04A23L33/125
CPCC12P19/04A23L33/125A23V2002/00A23V2200/30A23V2250/51
Inventor 王敏宋佳苏艳郑宇范冰倩夏婷申雁冰骆健美
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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