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A kind of extraction method of hemp seed globulin and its product and application

An extraction method, the technology of hemp seed, which is applied in the field of hemp seed globulin extraction, can solve the problems of relatively little extraction process research, and achieve the effects of high purity, prevention of cardiovascular diseases, and simple and easy-to-operate extraction method

Active Publication Date: 2022-04-19
YUNNAN HEMPMON PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, there are many reports about the functionality of hempseed globulin, but there are relatively few studies on its extraction process. Therefore, a simple and easy to operate, high globulin yield, high purity and good functionality have been developed. The method of extracting edestin is very meaningful

Method used

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  • A kind of extraction method of hemp seed globulin and its product and application
  • A kind of extraction method of hemp seed globulin and its product and application
  • A kind of extraction method of hemp seed globulin and its product and application

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Embodiment 1

[0098] The present embodiment provides a kind of extraction method of hemp seed globulin, described method is as follows:

[0099] (1) carry out dehydration and deoiling treatment to hemp seed, make its moisture content be 8%, oil content rate is 4%;

[0100] (2) Mix 100 g of treated hemp seeds with saline solution at a mass volume ratio of 1:10 g / mL, first heat and extract at 70 °C for 30 min, and then ultrasonically extract at 35 °C for 120 min, with an ultrasonic power of 800 W / L to obtain an extract; the salt solution is a salt solution system composed of 5% sodium chloride, 2% ammonium sulfate, 0.5% hyaluronic acid and 0.5% trehalose.

[0101] (3) filtering the extract obtained in step (2), then centrifuging to obtain a supernatant;

[0102] (4) The supernatant obtained in step (3) was left standing and cooled to 4°C, the protein was precipitated, centrifuged, washed at 4°C with 1% aqueous methylcellulose solution, and then washed with 90% aqueous ethanol at 4°C. After ...

Embodiment 2

[0104] The present embodiment provides a kind of extraction method of hemp seed globulin, described method is as follows:

[0105] (1) carry out dehydration and deoiling treatment to hemp seed, make its moisture content be 5%, oil content rate 5%;

[0106] (2) Mix 100g of processed hemp seeds with saline solution at a mass volume ratio of 1:5g / mL, first heat and extract at 60°C for 40min, then ultrasonically extract at 40°C for 60min, and the ultrasonic power is 800W / L to obtain an extract; the salt solution is a salt solution system composed of 6% sodium chloride, 1% ammonium sulfate and 1% hyaluronic acid.

[0107] (3) filtering the extract obtained in step (2), then centrifuging to obtain a supernatant;

[0108] (4) The supernatant obtained in step (3) was left standing and cooled to 4°C, the protein was precipitated, centrifuged, washed at 4°C with 0.5% aqueous methylcellulose, and then washed with 75% aqueous ethanol at 4°C After washing, 43.7 g of the hemp seed globuli...

Embodiment 3

[0110] The present embodiment provides a kind of extraction method of hemp seed globulin, described method is as follows:

[0111] (1) the hemp seed is carried out dehydration deoiling treatment, makes its water content be 10%, and oil content is 3%;

[0112] (2) Mix 100g of processed hemp seeds with saline solution at a mass volume ratio of 1:15g / mL, first heat and extract at 80°C for 20min, then ultrasonically extract at 30°C for 180min, and the ultrasonic power is 800W / L to obtain an extract; the salt solution is a salt solution system composed of 3% sodium chloride, 4% ammonium sulfate and 1% trehalose.

[0113] (3) filtering the extract obtained in step (2), then centrifuging to obtain a supernatant;

[0114] (4) The supernatant obtained in step (3) was left standing and cooled to 4°C, the protein was precipitated, centrifuged, washed at 4°C with 2% aqueous methylcellulose, and then washed with 95% aqueous ethanol at 4°C After washing, 44.7 g of the hemp seed globulin w...

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Abstract

The invention relates to a method for extracting hemp seed globulin and its product and application. The extraction method comprises the steps of dehydrating and deoiling the hemp seed, mixing it with a salt solution, extracting, filtering and cooling to obtain the hemp seed globulin. The extraction method involved in the present invention is simple and easy to operate, and the extracted globulin has high yield and high purity, and contains all essential amino acids for the human body, especially arginine content, which can be applied to food, health care products or medicines, and has anti-inflammatory properties. fatigue, improve immune health, and prevent cardiovascular disease.

Description

technical field [0001] The invention belongs to the technical field of protein extraction technology, and in particular relates to an extraction method of hemp seed globulin and its product and application. Background technique [0002] Hemp (hemp, thread hemp) belongs to industrial hemp and has a long history of planting in my country. Hemp does not have the value of drug use, and its seeds have high edible value and can also be used as medicine. Hemp seed has the same source of medicine and food, and its medicinal effect was first seen in "Shen Nong's Materia Medica". In recent years, hemp seed has been widely used as health food and clinical medicine in my country. [0003] Hemp seed is rich in oil (30%-50%), the unsaturated fatty acid content contained in oil is higher than 85%, and the ratio of linoleic acid to linolenic acid, omega-6 to omega-3, is between 2.29-4.68 It is beneficial to human health; the main components of human brain tissue are lipids, of which abou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C07K1/36C07K1/34C07K1/30A61K38/01A61P39/00A61P9/00
CPCC07K14/415A61K38/011A61P39/00A61P9/00Y02P20/54
Inventor 赵兵徐鹏伟赵庆生袁晓凡常坦然于朝晖
Owner YUNNAN HEMPMON PHARMA CO LTD
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