Application of genkwanin in preparation of product for preventing and treating osteoporosis

A technology of osteoporosis and genkwain, which is applied in the field of medicine, can solve the problems of destroying the bone balance of elderly women, and achieve the effect of enhancing the killing activity

Pending Publication Date: 2020-11-27
SHANGHAI UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Postmenopausal osteoporosis (PMO) is a common bone disease characterized by excessive bone resorption and disrupts bone balance in elderly women. At present, many anti-osteoporosis drugs have positive effects on bone health, but are often accompanied by severe side effects, such as osteonecrosis of the jaw caused by bisphosphonates and the risk of liver cancer increased by calcitonin, so the application of genkwain in the preparation of products for the prevention and treatment of osteoporosis is proposed to solve the above-mentioned problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of genkwanin in preparation of product for preventing and treating osteoporosis
  • Application of genkwanin in preparation of product for preventing and treating osteoporosis
  • Application of genkwanin in preparation of product for preventing and treating osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Reagents and methods

[0023] (1) Reagents

[0024] Genkwain (HPLC≥98%) was purchased from Chengdu Desite (ESTIE) Biotechnology Co., Ltd., α-MEM and serum (FBS) were purchased from Thermo Fisher Scientific, RANKL and M-CSF were purchased from R&D The TRAP staining kit was purchased from Zhejiang Zhongji Biological Co., Ltd., the PCR primers were designed and synthesized by Nanjing GenScript Biotechnology Co., Ltd., and the cytoskeleton staining agent Phalloidin-iFluor 488 Reagent (ab176753) was purchased from Abcam (Cambridge, UK). , UK) and Prime Script RT Master Mix (#RR036A) and TB GreenPremix Ex Taq (RR420A) were purchased from Japan Takara Bio Inc (Shiga Prefecture, Japan).

[0025] (2) Experimental method

[0026] 1. Cell culture

[0027] Take C57BL / 6 mice aged 4-6 weeks, kill them by cervical dislocation, and aseptically separate the bilateral tibia and femur; wash the sterile cavity with serum-free α-MEM medium until it turns white, and blow off the cell c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of medicines, discloses application of genkwanin in preparation of a medicine for preventing and treating osteoporosis, also discloses application of the genkwanin in preparation of health products for preventing and treating osteoporosis, and further provides an in-vitro action mechanism of the genkwanin. The invention proves that the genkwanin can prevent mouse bone loss caused by ovariectomy. Previous evidence indicates that the genkwanin has an anti-inflammatory effect. Reports indicate that the genkwanin can significantly enhance the killing activity of natural killer cells and the cytotoxic activity of T lymphocytes. The invention proves that the genkwanin has an immunomodulatory effect, the antibacterial activity, plasmodium resistance, free radical scavenging activity and chemical prevention activity of the genkwanin are reported, the genkwanin is directly used as the medicine, the potential value of the genkwanin in a medicine for treating osteoporosis caused by female menopause is proved, and effective treatment guidance is provided for osteoporosis caused by female menopause.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of genkwain in the preparation of products for preventing and treating osteoporosis. Background technique [0002] Bone is composed of an organic protein matrix formed by calcium and phosphate crystals and inorganic hydroxyapatite. It is repaired by osteoblast-mediated bone formation and osteoclast-mediated bone resorption. However, the difference between formation and resorption is Imbalances between them can lead to many diseases including rheumatoid arthritis, periodontitis and aseptic loosening of orthopedic implants. [0003] Postmenopausal osteoporosis (PMO) is a common bone disease characterized by excessive bone resorption and disrupts bone balance in elderly women. At present, many anti-osteoporosis drugs have positive effects on bone health, but are often accompanied by severe side effects, such as osteonecrosis of the jaw caused by bisphosphonates and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P19/10
CPCA61K31/352A61P19/10Y02A50/30
Inventor 苏笠黄标通李英华富欣张晨曦
Owner SHANGHAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap