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Adipose tissue composite preparation, and preparation method and application thereof

A technology of adipose tissue and compound preparations, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as slow growth of new blood vessels, slow blood supply reconstruction, and delayed blood supply, so as to promote blood vessel formation and tissue repair, and promote Effects of blood vessel formation and improvement of survival rate

Inactive Publication Date: 2016-12-21
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth of new blood vessels is slow, only growing more than ten microns per day, resulting in slow reconstruction of peripheral blood supply of transplanted fat, delayed blood supply, and long-term ischemia in the center of transplanted fat

Method used

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  • Adipose tissue composite preparation, and preparation method and application thereof
  • Adipose tissue composite preparation, and preparation method and application thereof
  • Adipose tissue composite preparation, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, condition screening test of adipose tissue compound preparation

[0059] The composite preparation in this example consists of adipose tissue, umbilical cord mesenchymal stem cells, SVF and PDGF. In order to determine the optimal range of the amount of each component, the condition screening test was carried out on the compound preparation with the wet weight as the index. The umbilical cord mesenchymal stem cells, SVF and PDGF are prepared into a cell suspension of suitable concentration with physiological saline, and the adipose tissue and the cell suspension are mixed evenly according to the volume ratio of 20:1, so that the composition of each component in the composite preparation of the present invention The final concentrations are shown in Table 1.

[0060] Table 1. Screening combinations of ingredients in compound preparations

[0061] group

Composition of compound preparation

control group

Adipose tissue

Experimen...

Embodiment 2

[0068] Embodiment 2, adipose tissue compound preparation and effect verification

[0069] The composite preparation in this example consists of adipose tissue, umbilical cord mesenchymal stem cells, SVF and PDGF. In order to further verify the effect of the compound preparation of the optimal formula, the umbilical cord mesenchymal stem cells, SVF and PDGF were prepared with normal saline to make a cell suspension of appropriate concentration, and the adipose tissue and the cell suspension were mixed evenly at a volume ratio of 20:1 , so that the final concentration of each component in the compound preparation of the present invention is as shown in Table 3.

[0070] Table 3. Grouping for the verification of the combination effect of each component in the compound preparation

[0071]

[0072]

[0073] A total of 120 females were selected for 4-6 weeks and were randomly divided into 6 groups, 20 in each group. Anesthetized by intraperitoneal injection of 2% pentobarbi...

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Abstract

The invention relates to an adipose tissue composite preparation, and a preparation method and an application thereof. The adipose tissue composite preparation includes PDGF, umbilical cord mesenchymal stem cells, SVF and adipose tissues. The preparation method comprises the following steps: preparing a cell suspension from the SVF, the umbilical cord mesenchymal stem cells and the PDGF, and uniformly mixing the adipose tissues with the cell suspension according to a volume ratio of 20:1 in order to prepare the adipose tissue composite preparation. The adipose tissue composite preparation can be applied in plastic surgery or beauty treatment as a filling preparation. The composite biological preparation has the advantages of easiness in revascularization after being injected, and increased survival rate, is safe and reliable, allows the adipose tissues to be pale yellow, soft and bright and to be only wrapped with few fibers after being transplanted for 2-3 months; and the density of capillary tubes in the center position of transplanted fats is normal, and the blood vessel revascularization is obvious.

Description

technical field [0001] The invention relates to the field of biological preparations, in particular to an adipose tissue composite preparation and its preparation method and application. Background technique [0002] Autologous fat grafting is a fat grafting solution often used in clinic. The advantages of this method are good biocompatibility, abundant tissue sources, easy shaping, and no adverse reactions such as immune rejection; the disadvantage is that it is injected into the face or breasts The fat particles in the area cannot establish a blood supply system well. When a large amount of fat accumulates together, the fat cells will be liquefied, necrotic, dissolved, and absorbed due to insufficient blood supply, which can easily cause sequelae such as infection, deformation, and pain, and often require re-injection to maintain the shape. [0003] The Chinese invention patent application with the application number 201110203210.7 discloses a preparation method of adipos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/36A61L27/22A61L27/38
CPCA61L27/54A61L27/3604A61L27/3625A61L27/3834A61L2300/30A61L2300/412A61L2300/414A61L2300/426A61L2430/00A61L2430/04
Inventor 陈海佳葛啸虎王一飞仇欣霞王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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