Industrial method for producing cannabinoid compound by chromatographic separation

A chromatographic separation and compound technology, which is applied in the industrial field of production of cannabinoid compounds by chromatographic separation, can solve the problems of limiting the development and utilization of active ingredients

Active Publication Date: 2020-12-01
CHENGUANG BIOTECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there are no reports in the prior art on the simultaneous extraction and separation of three different cannabinoid components from

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Step 1, use ethanol to extract and concentrate the crude cannabis extract, weigh 100g of the crude cannabis extract, dilute and dissolve it with 1000mL70% ethanol, if necessary, use a 50°C water bath to promote dissolution, and filter it with a 200-mesh filter cloth after dissolution to obtain the above column liquid.

[0047] Step 2, washing miscellaneous. Put the upper column liquid into the preparative chromatography, wash 2BV with 70% ethanol;

[0048] Step three: Elution. Elute 4BV with 80% ethanol, the first 1BV is impurity, the 1st-2BV is cannabidiol solution, and the 2.5-4BV is cannabidiol solution; Hydrocannabinol solution, after 2BV is regenerative filler.

[0049] Step 3: Concentrate the different effluents collected in step 2 until there is no condensate, and then use the deodorization pot to deodorize until the residual solution is less than 50ppm, and you can obtain cannabidiol oil and cannabis with a purity of more than 95%. Diphenol oil and THC oil p...

Embodiment 2

[0051] Step 1: Use ethanol to extract and concentrate the crude cannabis extract, weigh 150g of the crude cannabis extract, dilute and dissolve it with 3000mL70% ethanol, if necessary, use a 50°C water bath to promote dissolution, and filter it with a 200-mesh filter cloth after dissolution to obtain the above column liquid.

[0052] Step 2, put the upper column liquid into the preparative chromatography, wash impurities: first wash 2BV with 70% ethanol; elution: then use 85% ethanol to elute 4BV, the first 1BV is impurities, and the first 2BV is cannabidiol Solution, the 2.5-3.5BV is the cannabidiol solution; Elution: Then 4BV is eluted with 95% ethanol, the first 2BV is the tetrahydrocannabinol solution, and the last 2BV is the regeneration filler.

[0053] Step 3: Concentrate the different effluents collected in step 2 until there is no condensate, and then use the deodorization pot to deodorize until the residual solution is less than 50ppm, and you can obtain cannabidiol ...

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PUM

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Abstract

The invention relates to a chromatographic separation method of a cannabinoid compound, which comprises chromatographic separation on cannabis sativa extract by adopting an ethanol aqueous solution, and comprises the following steps: (1) washing for impurity removal: washing impurities by adopting a 50-70 (volume)% ethanol aqueous solution of 2BV, (2) gradient elution: adopting 75%-95% (volume) ofan ethanol aqueous solution as a gradient elution solvent, and respectively collecting an eluent of the first BV to the second BV, an eluent of the 2.5th to the fourth BV and an eluent of the fourthBV to the sixth BV, and (3) concentration: respectively concentrating the eluents collected in the step (2). By adopting the method disclosed by the invention, the high-purity hypocannabidiol, cannabidiol and tetrahydrocannabinol can be directly separated, and the purity of the hypocannabidiol, cannabidiol and tetrahydrocannabinol reaches up to 95% or above after the eluent is concentrated.

Description

technical field [0001] The invention relates to the technical field of preparation of various cannabinoids in industrial hemp, in particular to an industrial extraction method of cannabidiol, cannabidiol and tetrahydrocannabinol. Background technique [0002] Cannabis is an annual herb of Cannabis genus Cannabis, which has important agricultural and medicinal value. Marijuana contains a toxic component THC, which can make people hallucinogenic and addictive, and can be used as a drug. It has been banned for a long time. [0003] At present, there are large-scale cultivations of hemp raw materials exclusively for industrial use. The tetrahydrocannabinol (THC) content in the flowers and leaves during the growth period is less than three per thousand, which does not have the value of extracting the toxic component THC or directly as a drug. Smoking, can be legally carried out large-scale planting and industrial development and utilization. [0004] The cannabinoids contained ...

Claims

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Application Information

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IPC IPC(8): C07C39/23C07C37/82C07C37/68C07D311/80
CPCC07C37/82C07C37/68C07D311/80C07C2601/16C07C39/23
Inventor 高伟李杨刘登帅田洪袁红帅
Owner CHENGUANG BIOTECH GRP CO LTD
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