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Method for researching migration process of steroid estrogen in wheat

A migration process, estrogen technology, applied in the field of pollution phytoremediation, to achieve the effect of low design cost, simple process and convenient operation

Active Publication Date: 2020-12-01
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have not reported whether steroid estrogen can migrate from aerial parts to roots.

Method used

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  • Method for researching migration process of steroid estrogen in wheat
  • Method for researching migration process of steroid estrogen in wheat
  • Method for researching migration process of steroid estrogen in wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Soak wheat seeds in 10%, 15% and 20% hydrogen peroxide for 10 minutes, wash thoroughly with deionized water, select uniform and plump seeds, place them on wet filter paper, and germinate them in the dark for 5 days. Keep the filter paper moist at all times. After the seeds germinate, they are placed in a petri dish containing 1 / 4 standard concentration of Hoagland's nutrient solution, and cultivated under the set culture conditions until the seedlings grow until the fourth true leaf appears. Then place it in a petri dish containing 1000 μg / L 17β-E2 solution, and add nutrient solution to the solution at the same time, so that the concentration is 1 / 2 of the standard Hoagland nutrient solution concentration to ensure normal growth of wheat. After 7 days of culture, destructive sampling was carried out, and the roots of wheat seedlings were taken to determine the content of 17β-E2. During the cultivation process, the cultivation conditions of wheat seedlings were in addit...

Embodiment 2

[0048] The plant culture device is composed of two 150mL glass chambers and a cover sheet. The cover sheet is a cardboard with a diameter of 1cm in the middle and a thickness of 3cm. The hole can ensure that the roots are divided into two parts evenly. Wheat roots enter the two glass chambers under the cover respectively, and a certain thickness can keep the wheat seedlings growing upright; the two glass chambers under the cover leave suitable gaps to prevent mutual contamination of the culture medium.

Embodiment 3

[0050] Wheat seeds were treated with 15% H 2 o 2 After soaking and disinfecting for 10 minutes, wash thoroughly with deionized water, select uniform and plump seeds, place them on wet filter paper, and germinate them in the dark for 5 days, keeping the filter paper moist throughout the germination period. After the seeds germinate, they are placed in a petri dish containing 1 / 4 standard concentration of Hoagland's nutrient solution, and cultivated under the set culture conditions until the seedlings grow until the fourth true leaf appears. Add 50 mL of deionized water and 50 mL of 1000 μg / L 17β-E2 solution to the two chambers of the plant culture device, and simultaneously add the same volume of Hoagland’s nutrient solution of standard concentration to the two containers to make the concentration It is 1 / 2 of the concentration of the standard Hoagland nutrient solution to ensure the normal growth of wheat. Take wheat seedlings with similar growth after being cultured in the ...

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Abstract

The invention discloses a method for researching the migration process of steroid estrogen in wheat, which belongs to the technical field of remediation of polluted plants in water or soil. The methodcomprises the steps of 1) respectively adding nutrient solutions containing 17 beta-E2 and not containing 17 beta-E2 into different cavities of a plant culture device; 2) equally dividing the root system of the mature root system seedling into two parts, and respectively transplanting the two parts into a chamber of a plant culture device; and 3) respectively measuring the contents of 17 beta-E2in the overground part, the root system part and the nutrient solution of the seedling at different culture time. By sampling and measuring at different time points, the cumulants of 17beta-E2 at different positions of the plant along with time can be visually seen, so that the migration process of 17beta-E2 in the plant body is judged. The method is simple in technological process, convenient tooperate, free of complex experimental equipment and low in design cost, and provides important technical support for researching the migration accumulation process of steroid estrogens and other pollutants in plants.

Description

technical field [0001] The invention belongs to the technical field of phytoremediation of polluted water or soil, and in particular relates to a research method for the migration process of steroid estrogen in wheat. Background technique [0002] Steroid estrogen is mainly a hormone substance produced by human or animal ovarian cells. When it enters the animal body as an exogenous estrogen, it can simulate the action of the endogenous hormone in the cell or change its activity, thereby affecting human and animal sex. Differentiation and normal metabolic functions in the body interfere with the normal growth, development, reproduction and other functions of organisms, and even produce lethal, carcinogenic and teratogenic effects. They are new micro-pollutants that have attracted much attention in the 21st century. Studies have shown that steroid estrogens in very low concentration levels (such as 1ng / L -1 ) will cause harm to living organisms. The effluent of sewage treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N33/00B09C1/00B09C1/10
CPCB09C1/002B09C1/105G01N30/02G01N33/0098
Inventor 李艳霞陈兴财
Owner BEIJING NORMAL UNIVERSITY