An integrated in vitro surrogate model of human gastrointestinal digestion and absorption

Abstract

The invention provides an integrated human gastrointestinal digestion and absorption in vitro substitution model, which is integrated by 12 systems into an integrated in vitro simulation digestion model, including a gastric digestion simulation system, a small intestine digestion simulation system, a small intestine absorption simulation system, a large intestine Decomposition simulation system, central intelligent control system, pH control system, temperature control system, stirring rate control system, conveying system, central purification system, automatic cleaning and disinfection system and anaerobic system. Through the synergy of various systems, this in vitro surrogate model not only simulates the digestion and absorption process of the gastrointestinal tract in vitro, but also simulates the fermentation and decomposition process of the intestinal microbial community. This model is suitable for accurately quantifying the metabolic process and toxic effect of nutrients or pollutants in the food matrix in the body, and obtaining relevant data such as bioaccessibility, bioavailability, metabolic fate and interaction with microorganisms of nutrients or pollutants. Realize precise risk assessment.

Description

technical field [0001] The invention belongs to the technical fields of food safety, in vitro substitution toxicology, microbiology, nutrition, organic chemistry and the like, and in particular relates to an integrated human gastrointestinal digestion and absorption in vitro substitution model. Background technique [0002] After the food enters the gastrointestinal tract of the human body through the mouth, the nutrients or pollutants combined with the food matrix are released into the gastrointestinal juice under the digestion of the gastrointestinal tract, absorbed through the digestive tract, and finally pass through the human body such as blood or lymphatic tissue Circulation reaches the target organ to form a healthy effect. This series of processes is a complex process of digestive juice secretion, enzyme reaction, gastrointestinal motility, and intestinal microbial community. However, the combination form, structure, and physical and chemical properties of nutrients ...

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Problems solved by technology

The above invention patents have established related in vitro digestion models for simulating human intestinal microorganisms and simulating human gastrointestinal digestion and intestinal microecology, but they also failed to simulate the absorption mechanism, immune mechanism and microbial metabolism mechanism of the digestive tract. simulation of

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