Hydrogel microcarrier and its preparation method and application

A hydrogel and microcarrier technology, applied in the field of biomedical detection, can solve problems such as complex detection steps, achieve low detection cost, high detection sensitivity, and reduce decoding costs
CN112048033BActive Publication Date: 2021-10-29HUNAN UNIV
3 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUNAN UNIV
Publication Date
2021-10-29

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a hydrogel microcarrier and its preparation method and application. The hydrogel microcarrier uses an oil phase solution as a carrier, and an aqueous phase solution containing an initiator and two or more colloidal materials is dispersed in the In the oil phase solution, by changing the concentration ratio of different gel-forming materials, a density-coded hydrogel microcarrier is formed. Its preparation method is to mix two or more gel-forming materials with different concentration ratios and initiators to obtain a water phase solution; dissolve the surfactant and TEMED in an oil medium to obtain an oil phase solution, and wrap the water phase solution in oil In the phase solution, hydrogel beads are formed, and the hydrogel beads are solidified into density-coded hydrogel microcarriers. The hydrogel microcarrier of the present invention can realize multiple detection of disease-related proteins and visual detection of tumor-derived exosomes, and has the advantages of simple decoding and low detection cost.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biomedical detection, in particular to a hydrogel microcarrier and its preparation method and application. Background technique

[0002] With the rapid development of biomedicine, high-throughput biological analysis is required in the fields of disease detection and drug discovery. Although planar chip technology is widely used in high-throughput analysis, they have certain limitations in reaction speed, repeatability and reliability. Suspension array is another alternative strategy, and the probe molecular carrier can be freely dispersed into the reaction system without being limited by the space position.

[0003] For suspended arrays, there are mainly two key issues. One is the need to encode the mobile vector. In recent years, various new coding vectors have emerged and been widely used. Among them, polymer microspheres have significant advantages as a solid phase carrier, such as large specific surface are...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More