Hydrogel microcarrier and its preparation method and application

A hydrogel and microcarrier technology, applied in the field of biomedical detection, can solve problems such as complex detection steps, achieve low detection cost, high detection sensitivity, and reduce decoding costs

Active Publication Date: 2021-10-29
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, many methods for detecting tumor-derived exosomes have been developed, but most of these methods require the cooperation of large-scale instruments, and the detection steps are relatively complicated

Method used

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  • Hydrogel microcarrier and its preparation method and application
  • Hydrogel microcarrier and its preparation method and application
  • Hydrogel microcarrier and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] A kind of hydrogel microcarrier of the present invention, its preparation method comprises the following steps:

[0076] (1) Preparation of water phase precursor solution and oil phase precursor solution:

[0077] 1.1. Preparation of aqueous precursor solution:

[0078] PEGDA monomer (20%, w / v), APS (0.3%, w / v) and MAA (0%, w / v) were mixed to obtain aqueous phase precursor solution A1.

[0079] Mix PEGDA monomer (20%, w / v), APS (0.3%, w / v) and MAA (10%, w / v) to obtain aqueous phase precursor solution A2;

[0080] Mix PEGDA monomer (20%, w / v), APS (0.3%, w / v) and MAA (20%, w / v) to obtain aqueous phase precursor solution A3;

[0081] PEGDA monomer (20%, w / v), APS (0.3%, w / v) and MAA (30%, w / v) were mixed to obtain aqueous phase precursor solution A4.

[0082] 1.2. Preparation of oil phase precursor solution: 2% (w / w) FSL surfactant and 0.4% (v / v) TEMED were dissolved in HFE-7500 oil.

[0083] (2) Preparation of hydrogel beads: Mix 0.25 mL of aqueous phase precursor so...

Embodiment 2

[0088] A kind of hydrogel microcarrier of the present invention, its preparation method comprises the following steps:

[0089] (1) Preparation of water phase precursor solution and oil phase precursor solution:

[0090] 1.1. Preparation of aqueous precursor solution:

[0091] PEGDA monomer (20%, w / v), APS (0.3%, w / v) and gelatin (0%, w / v) were mixed to obtain aqueous phase precursor solution B1.

[0092] PEGDA monomer (20%, w / v), APS (0.3%, w / v) and gelatin (2%, w / v) were mixed to obtain aqueous phase precursor solution B2.

[0093] PEGDA monomer (20%, w / v), APS (0.3%, w / v) and gelatin (4%, w / v) were mixed to obtain aqueous phase precursor solution B3.

[0094] 1.2. Preparation of oil phase precursor solution: 2% (w / w) FSL surfactant and 0.4% (v / v) TEMED were dissolved in HFE-7500 oil.

[0095] (2) Preparation of hydrogel beads: 0.25 mL of aqueous phase precursor solutions B1, B2, B3 and 0.5 mL of oil phase precursor solution were mixed and stirred at room temperature at 8...

Embodiment 3

[0100] A kind of hydrogel microcarrier of the present invention, its preparation method comprises the following steps:

[0101] (1) Preparation of water phase precursor solution and oil phase precursor solution:

[0102] 1.1. Preparation of aqueous precursor solution:

[0103] Silk fibroin (20%, w / v), APS (0.3%, w / v) and gelatin (0%, w / v) were mixed to obtain aqueous phase precursor solution C1.

[0104] Silk fibroin monomer (20%, w / v), APS (0.3%, w / v) and gelatin (2%, w / v) were mixed to obtain aqueous phase precursor solution C2.

[0105] 1.2. Preparation of oil phase precursor solution: 2% (w / w) FSL surfactant and 0.4% (v / v) TEMED were dissolved in HFE-7500 oil.

[0106] (2) Preparation of hydrogel beads: 0.25 mL of aqueous phase precursor solutions C1 and C2 and 0.5 mL of oil phase precursor solution were mixed, and stirred at room temperature at 800 rpm for 5 minutes to form a reverse phase suspension.

[0107] (3) Continue to polymerize the reverse phase suspension at ...

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Abstract

The invention discloses a hydrogel microcarrier and its preparation method and application. The hydrogel microcarrier uses an oil phase solution as a carrier, and an aqueous phase solution containing an initiator and two or more colloidal materials is dispersed in the In the oil phase solution, by changing the concentration ratio of different gel-forming materials, a density-coded hydrogel microcarrier is formed. Its preparation method is to mix two or more gel-forming materials with different concentration ratios and initiators to obtain a water phase solution; dissolve the surfactant and TEMED in an oil medium to obtain an oil phase solution, and wrap the water phase solution in oil In the phase solution, hydrogel beads are formed, and the hydrogel beads are solidified into density-coded hydrogel microcarriers. The hydrogel microcarrier of the present invention can realize multiple detection of disease-related proteins and visual detection of tumor-derived exosomes, and has the advantages of simple decoding and low detection cost.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a hydrogel microcarrier and its preparation method and application. Background technique [0002] With the rapid development of biomedicine, high-throughput biological analysis is required in the fields of disease detection and drug discovery. Although planar chip technology is widely used in high-throughput analysis, they have certain limitations in reaction speed, repeatability and reliability. Suspension array is another alternative strategy, and the probe molecular carrier can be freely dispersed into the reaction system without being limited by the space position. [0003] For suspended arrays, there are mainly two key issues. One is the need to encode the mobile vector. In recent years, various new coding vectors have emerged and been widely used. Among them, polymer microspheres have significant advantages as a solid phase carrier, such as large specific surface are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F122/20C08F222/20C08F289/00C08F220/56C08F2/32C08J3/075G01N33/58G01N33/68G01N33/574G01N33/569G01N33/53G01N21/64C08L35/02C08L33/26C08L51/08C08L89/00
CPCC08F2/32C08F122/20C08F289/00C08J3/075C08J2333/26C08J2335/02C08J2351/08C08J2389/00C08J2489/00C08F222/102G01N21/64G01N33/5308G01N33/56966G01N33/57434G01N33/57473G01N33/57484G01N33/582G01N33/68G01N33/74C08F220/56
Inventor 贺建军施李杨侯敏
Owner HUNAN UNIV
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