Young mango extract and application of compound obtained from young mango extract in skin care and health care
An extract and mango technology, applied in the field of plant extracts, can solve problems such as harmful skin health, achieve the effects of delaying aging, avoiding the loss of normal structure and function, and inhibiting protein glycation
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Embodiment 1
[0053] Preparation of Young Mango Fruit Extract
[0054] The mango (Mangifera indica) mentioned in this article refers to the mango variety produced in Taiwan, China, but not limited thereto. Generally speaking, the growth and development of mango fruit is divided into four stages, which are (1) young fruit stage: after the mango flower fades, the fruit begins to grow slowly and turns green; Starch gradually accumulates; (3) Maturity period: the fruit enters the maturity period after the endocarp hardens. At this time, the appearance of the fruit does not change much, but the weight of the fruit continues to increase, and some physical and chemical changes are still in progress. The hardness decreases, the sugar content increases, and the peel turns yellow, making the fruit close to the ripe and edible stage; (4) aging period: the fruit begins to age after it is fully ripe. The young mango fruit or immature mango fruit described herein refers to the mango fruit that has not e...
Embodiment 2
[0058] Young mango fruit extract inhibits melanin formation
[0059] In order to examine the effect of the young mango fruit extract on melanin production, the melanin content of the melanoma cell line B16F10 treated with the young mango fruit extract described in Example 1 was measured by a melanin production test. Briefly, B16F10 cells were cultured at 1.5×10 5Cells / well were seeded in 6-well culture plates, and each well contained 3 mL of DMEM cell culture medium. After culturing the cells at 37°C for 24 hours, the cell culture medium was removed, and 3 mL of DMEM cell culture medium containing 0.25 mg / mL mango young fruit extract (experimental group) or 0.25 mg / mL kojic acid (positive control group) was added Add cells to each well. In addition, a blank control group was set up, and the cells were only treated with 3 mL of DMEM cell culture medium. After culturing at 37°C for 48 hours, the aforementioned three groups of cells were collected to measure the melanin conten...
Embodiment 3
[0062] Young mango fruit extract prevents and reduces the death of dermal fibroblasts induced by ultraviolet A radiation
[0063] In order to test the effect of young mango fruit extract on protecting the skin against ultraviolet radiation, human dermal fibroblasts CCD-966SK were pre-treated with the young mango fruit extract described in Example 1 and then irradiated with ultraviolet A ( UVA protection test), or the cell survival rate of being treated with the young mango fruit extract described in Example 1 (UVA damage repair test) after ultraviolet A irradiation.
[0064] 3.1 UVA protection test
[0065] CCD-966SK cells were divided into 5×10 3 Cells / well were seeded in a 96-well culture plate, and each well contained 200 μL of MEM cell culture medium. After culturing the cells at 37°C for 24 hours, remove the cell culture medium, add 200 μL of MEM cell culture medium without or with 0.5 mg / mL young mango fruit extract to the cells in each well, and then culture the cells...
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